Abstract Minimal natural resistance in guar against Alternaria cyamopsidis blight or leaf spots requires the need to develop improved cultivars with high level of resistance. In this study, we report the silencing of AcCHS7 gene with pSilentCHS7-2 and pARTCHS7 constructs in Alternaria pathogen and guar plants of HG 2–20 variety, respectively. The down-regulation of AcCHS7 delays the disease progression in guar by damaging the hyphae, reducing mycelium growth and conidiation in Alternaria. Hence, this study demonstrates the role of host induced post-transcriptional gene silencing of AcCHS7 as an effective approach to increase the guar productivity by developing resistance against Alternaria cyamopsidis.

中文翻译：

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通过RNAi介导的瓜尔胶中几丁质合酶输出伴侣CHS7基因沉默对蓝藻链格孢的工程抗性

摘要 瓜尔豆对链格孢霉疫病或叶斑病的最小天然抗性需要开发具有高抗性的改良品种。在这项研究中，我们分别报告了在 HG 2-20 品种的链格孢属病原体和瓜尔豆植物中 pSilentCHS7-2 和 pARTCHS7 构建体对 AcCHS7 基因的沉默。AcCHS7 的下调通过破坏菌丝、减少链格孢中的菌丝体生长和分生孢子来延缓瓜尔豆的疾病进展。因此，本研究证明了宿主诱导的 AcCHS7 转录后基因沉默作为一种有效方法的作用，可通过产生对链格孢菌的抗性来提高瓜尔豆生产力。