The increasing demand in rapid wound dressing and healing has promoted the development of intraoperative strategies, such as intraoperative bioprinting, which allows deposition of bioinks directly at the injury sites to conform to their specific shapes and structures. Although successes have been achieved to varying degrees, either the instrumentation remains complex and high-cost or the bioink is insufficient for desired cellular activities. Here, we report the development of a cost-effective, open-source handheld bioprinter featuring an ergonomic design, which was entirely portable powered by a battery pack. We further integrated an aqueous two-phase emulsion bioink based on gelatin methacryloyl with the handheld system, enabling convenient shape-controlled in situ bioprinting. The unique pore-forming property of the emulsion bioink facilitated liquid and oxygen transport as well as cellular proliferation and spreading, with an additional ability of good elasticity to withstand repeated mechanical compressions. These advantages of our pore-forming bioink-loaded handheld bioprinter are believed to pave a new avenue for effective wound dressing potentially in a personalized manner down the future.

对快速伤口敷料和愈合的需求不断增长，促进了术中策略的发展，例如术中生物打印，它允许将生物墨水直接沉积在损伤部位，以符合其特定的形状和结构。尽管已经取得了不同程度的成功，但仪器仍然复杂且成本高昂，或者生物墨水不足以实现所需的细胞活动。在这里，我们报告了一种具有成本效益的开源手持式生物打印机的开发，该打印机具有人体工程学设计，完全由电池组供电，便携式。我们进一步将基于明胶甲基丙烯酰的水性两相乳液生物墨水与手持系统集成，从而实现方便的形状控制原位生物打印。乳液生物墨水独特的成孔特性促进了液体和氧气的运输以及细胞增殖和扩散，并具有良好的弹性以承受重复的机械压缩。我们相信，我们的成孔生物墨水手持式生物打印机的这些优势将为未来以个性化方式有效伤口敷料铺平一条新途径。