This study investigated effect of various combinations of phytase, citric acid (CA), and vitamin D₃ (D₃) on phytate P utilization as evaluated by growth performance, serum minerals, and bone characteristics in broiler chickens fed the diet severely limited in non-phytate P. A total of 585 ten-day-old male broiler chickens were allocated to 9 diets from 11 to 38 d of age. There were a positive control (with adequate non-phytate P concentration and 2,000 IU D₃/kg in the diet) and 8 diets (without any inorganic P) consisting of D₃ (2,000 and 4,000 IU/kg), CA (0 and 15 g/kg), and phytase (0 and 1,500 FTU/kg) in a 2 × 2 × 2 factorial arrangement of treatments with 5 pens per treatment and 13 broiler chickens per pen. Phytase supplementation increased average daily gain and decreased feed to gain ratio, but the effect of phytase was more apparent in broiler chickens received CA (phytase × CA, P < 0.05 and P < 0.01 for average daily gain and feed to gain ratio, respectively). Serum P was increased by phytase supplementation and decreased by CA supplementation, but the decreasing effect of CA was not observed in broiler chickens received phytase (phytase × CA, P < 0.05). Addition of 4,000 IU D₃/kg increased breast muscle yield (P < 0.05) and decreased abdominal fat pad (P < 0.05). Phytase supplementation increased carcass yield (P < 0.05) and breast muscle yield (P < 0.001). The individual use of phytase increased bone ash percentage and the combination of 4,000 IU D₃/kg and CA increased it, but the inclusion of phytase, CA, and 4,000 IU D₃/kg simultaneously did not increase further bone ash percentage (phytase × CA × D₃, P < 0.05). Bone strength was increased by phytase supplementation and not affected by supplementation of CA or 4,000 IU D₃/kg, but the effect of phytase was more apparent in broiler chickens received CA or 4,000 IU D₃/kg; however, the inclusion of phytase, CA, and 4,000 IU D₃/kg simultaneously did not increase further bone strength (phytase × CA × D₃, P < 0.01). In conclusion, the combination of phytase and CA was more effective than other possible combinations in improving growth performance of broiler chickens fed a diet severely limited in non-phytate P.

这项研究调查了植酸酶，柠檬酸（CA）和维生素D ₃（D ₃）的各种组合对植酸磷利用率的影响，通过生长性能，血清矿物质和骨骼特性（在饮食中严格限制）的肉鸡的生长性能来评估植酸磷。共有585只10天大的雄性肉鸡被分配到11日至38天龄的9种日粮中。有一个阳性对照（饮食中有足够的非植酸磷浓度和2,000 IU D ₃ / kg）和8种饮食（不含任何无机P）由D ₃组成（2,000和4,000 IU / kg），CA（0和15 g / kg）和植酸酶（0和1,500 FTU / kg）以2×2×2因子处理排列，每种处理5支，每只处理13只肉鸡钢笔。补充植酸酶可增加平均日增重，降低饲料增重比，但是植酸酶的作用在接受CA的肉鸡身上更为明显（植酸酶×CA，平均每日增重和饲料增重比分别为P <0.05和P <0.01） 。补充植酸酶可增加血清P，而添加CA则可降低血清P，但在​​接受植酸酶的肉鸡中未观察到CA的降低作用（植酸酶×CA，P <0.05）。增加4,000 IU D ₃ / kg可增加乳房肌肉产量（P<0.05）和腹部脂肪垫减少（P <0.05）。补充植酸酶可提高car体产量（P <0.05）和胸肌产量（P <0.001）。单独使用肌醇六磷酸酶会增加骨灰分，而4,000 IU D ₃ / kg和CA的组合会增加骨灰分，但同时添加肌醇六磷酸酶，CA和4,000 IU D ₃ / kg并不会进一步增加骨灰分百分率。 CA×D ₃，P＜0.05）。补充肌醇六磷酸酶可增加骨骼强度，但不受CA或4,000 IU D ₃ / kg的影响，但植酸酶的作用在接受CA或4,000 IU D _3的肉鸡中更为明显/公斤; 但是，同时添加植酸酶，CA和4,000 IU D ₃ / kg并不能进一步提高骨强度（植酸酶×CA×D ₃，P <0.01）。总之，肌醇六磷酸酶和CA的组合在改善饲喂非肌醇六磷酸磷含量严格限制的肉鸡的生长性能方面比其他可能的组合更有效。