Invadosomes, which encompass podosomes and invadopodia, are actin rich adhesive and protrusive structures facilitating invasion and migration in various cell types. Podosomes are mostly found in normal cells, while invadopodia are hallmarks of invasive transformed cells. Despite evident structural differences, both structures mostly rely on the same pathways for their formation and their activity. While the role of actin cytoskeleton is undeniable, the involvement of microtubules (MTs) in invadosome formation/activity has recently been demonstrated but also somehow underestimated. MTs are components of the eukaryotic cytoskeleton well known for their essential roles for cell division, the maintenance of cell shape, intracellular transport and cell motility. Until now, MTs were mostly seen as railways for the delivery of various cargos required for invadosome functions but recent data suggest a more complex role. In this review, we address the specific functions of MTs on invadosome dynamics, activity, maturation and organization in light with recent data, which extended far beyond simple track delivery. Indeed, MT dynamic instability, which in turn modulates Rho GTPase signalling and likely MT post-translational modifications are playing major roles in invadosome functions.

侵袭小体包括足小体和侵袭足，是富含肌动蛋白的粘附和突出结构，促进各种细胞类型的侵袭和迁移。Podosomes 主要存在于正常细胞中，而 invadopodia 是侵入性转化细胞的标志。尽管存在明显的结构差异，但这两种结构的形成和活性大多依赖于相同的途径。虽然肌动蛋白细胞骨架的作用是不可否认的，但最近已经证明了微管 (MT) 参与侵入体形成/活动，但也以某种方式被低估了。MTs 是真核细胞骨架的组成部分，众所周知，它们在细胞分裂、维持细胞形状、细胞内运输和细胞运动方面发挥着重要作用。到现在，MTs 主要被视为用于运送入侵体功能所需的各种货物的铁路，但最近的数据表明其作用更为复杂。在这篇综述中，我们根据最近的数据解决了 MTs 在侵入体动力学、活动、成熟和组织方面的特定功能，这些数据远远超出了简单的跟踪传递。事实上，MT 动态不稳定性，反过来调节 Rho GTPase 信号和可能的 MT 翻译后修饰在侵袭体功能中发挥主要作用。