Arthrobacter sp. KFS-1 previously isolated from a dump site was used to produce keratinase in basal medium. The physico-chemical conditions were optimized to enhance the keratinase production, and biochemical properties of the enzyme were also evaluated. Arthrobacter sp. KFS-1 optimally produced keratinase in a basal medium that contained 1.0 g/L xylose, 2.5–5.0 g/L chicken feather; with initial pH, incubation temperature and agitation speed of 6.0, 30 °C and 200 rpm, respectively. Maximum keratinase activity of 1559.09 ± 29.57 U/mL was achieved at 96 h of fermentation; while optimal thiol concentration of 665.13 ± 38.73 μM was obtained at 144 h. Furthermore, the enzyme was optimally active at pH 8.0 and 60 °C. The enzyme activity was inhibited by ethylene diamine tetraacetic acid and 1,10-phenanthroline, but not affected by phenylmethylsulfonyl floride. In addition, the crude enzyme retained 55%, 63%, 80%, 81% and 90% of the original activity after respective pretreatment with some commercial detergents (Maq, Omo, Surf, Sunlight and Ariel). Moreso, the enzyme showed remarkable stability in the presence of reducing agents, surfactants, and organic solvents. Arthrobacter sp. KFS-1 significantly produced keratinase which exhibited excellent stability in presence of chemical agents and commercial laundry detergents; hence, suggesting its industrial application potentials especially in detergent formulation.

节藻。先前从垃圾场分离的KFS-1用于在基础培养基中产生角蛋白酶。优化了物理化学条件以增强角蛋白酶的产生，并且还评估了该酶的生化特性。关节杆菌sp。KFS-1在含有1.0 g / L木糖，2.5–5.0 g / L鸡毛的基础培养基中最佳产生角蛋白酶；初始pH，孵育温度和搅拌速度分别为6.0、30°C和200 rpm。发酵96小时达到的最大角蛋白酶活性为1559.09±29.57 U / mL；144 h时硫醇最佳浓度为665.13±38.73μM。此外，该酶在pH 8.0和60°C时具有最佳活性。乙二胺四乙酸和1,10-菲咯啉可抑制酶的活性，但不受苯基甲基磺酰氟的影响。另外，在分别用一些商业洗涤剂（Maq，Omo，Surf，Sunlight和Ariel）进行预处理后，粗酶保留了原始活性的55％，63％，80％，81％和90％。而且节藻。KFS-1可显着产生角蛋白酶，在化学试剂和市售洗涤剂的存在下具有优异的稳定性。因此，表明其工业应用潜力，特别是在洗涤剂配方中。