ABSTRACT: Knowledge of the anthropogenic drivers of environmental change is important for understanding ecosystem dynamics. We studied 2 of these factors—an invasive mollusk (golden mussel Limnoperna fortunei) and the herbicide glyphosate—focusing on the effects of the mussel, the herbicide, and their interaction on the abundance and composition of different groups of biofilm bacteria present on the mussel’s valves. We carried out semi-static experiments to assess changes in nutrient and glyphosate concentrations in the presence or absence of the mussel. The catalyzed reporter deposition-fluorescence in situ hybridization technique was used to evaluate changes in biofilm bacteria growing on whole mussels or dissected valves. When the mussel was exposed to glyphosate, there was a significant decrease in the concentration of the herbicide, a significant increase in the concentration of its major metabolite (aminomethylphosphonic acid), and a significant increase in the concentration of nutrients. These results may be explained by the capacity of biofilm bacteria associated with L. fortunei to degrade glyphosate. After exposure to the herbicide, the analysis of 5 groups of Eubacteria (i.e. Alpha-, Beta-, and Gammaproteobacteria, Actinobacteria, and Bacteroidetes) showed a significant increase in the abundance of Gammaproteobacteria, suggesting that they would participate in glyphosate transformation in water. This study represents a starting point for investigating the bacterial component of the biofilm present on the valves of L. fortunei. Moreover, this invasive mussel is a promising tool for glyphosate degradation. However, the liberation of nutrients as a consequence of the degradation of herbicide mediated by the presence of L. fortunei may accelerate eutrophication processes in freshwater ecosystems.

中文翻译：

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林木阀门上生物膜的细菌组成及其在水中草甘膦降解中的作用。

摘要：了解环境变化的人为驱动因素对于理解生态系统动态非常重要。我们研究了其中两个因素-侵入性软体动物（金贻贝Limnoperna fortunei）和除草剂草甘膦-着眼于贻贝，除草剂及其相互作用对贻贝上存在的不同种类生物膜细菌的丰度和组成的影响阀门。我们进行了半静态实验，以评估贻贝存在或不存在时营养素和草甘膦浓度的变化。催化的报告分子沉积-原位荧光杂交技术用于评估在整个贻贝或解剖的瓣膜上生长的生物膜细菌的变化。当贻贝接触草甘膦时，除草剂的浓度显着降低，其主要代谢物（氨基甲基膦酸）的浓度显着增加，营养素的浓度显着增加。这些结果可以通过与福氏乳杆菌相关的生物膜细菌降解草甘膦的能力来解释。暴露于除草剂后，分析5种真细菌（即Alpha，Beta和Gammaproteobacteria，Actinobacteria和Bacteroidetes））显示丙种细菌的丰度显着增加，表明它们将参与水中的草甘膦转化。这项研究是调查存在于的阀门生物膜细菌的菌体成分起点L.柳杉。此外，这种侵入性贻贝是用于草甘膦降解的有前途的工具。然而，由于福寿菌的存在介导的除草剂降解导致营养物质的释放可能会加速淡水生态系统的富营养化过程。