ABSTRACT: Various studies have observed that increased nutrient supply promotes the growth of bloom-forming cyanobacteria, but only a limited number of studies have investigated the influence of increased nutrient supply on bloom-forming cyanobacteria at the proteomic level. We investigated the cellular and proteomic responses of Microcystis aeruginosa to elevated nitrogen and phosphorus supply. Increased supply of both nutrients significantly promoted the growth of M. aeruginosa and the synthesis of chlorophyll a, protein, and microcystins. The release of microcystins and the synthesis of polysaccharides negatively correlated with the growth of M. aeruginosa under high nutrient levels. Overexpressed proteins related to photosynthesis, and amino acid synthesis, were responsible for the stimulatory effects of increased nutrient supply in M. aeruginosa. Increased nitrogen supply directly promoted cyanobacterial growth by inducing the overexpression of the cell division regulatory protein FtsZ. NtcA, that regulates gene transcription related to both nitrogen assimilation and microcystin synthesis, was overexpressed under the high nitrogen condition, which consequently induced overexpression of 2 microcystin synthetases (McyC and McyF) and promoted microcystin synthesis. Elevated nitrogen supply induced the overexpression of proteins involved in gas vesicle organization (GvpC and GvpW), which may increase the buoyancy of M. aeruginosa. Increased phosphorus level indirectly affected growth and the synthesis of cellular substances in M. aeruginosa through the mediation of differentially expressed proteins related to carbon and phosphorus metabolism. This study provides a comprehensive description of changes in the proteome of M. aeruginosa in response to an increased supply of 2 key nutrients.

中文翻译：

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在细胞和蛋白质组学水平上养分供应增加对铜绿微囊藻的影响

摘要：各种研究已经观察到养分供应的增加会促进水华形成蓝藻的生长，但是只有少数研究在蛋白质组学水平上研究了养分供应对水华形成蓝藻的影响。我们调查了铜绿微囊藻对氮和磷供应增加的细胞和蛋白质组学反应。两种营养素供应的增加都显着促进了铜绿假单胞菌的生长以及叶绿素a，蛋白质和微囊藻毒素的合成。微囊藻毒素的释放和多糖的合成与铜绿假单胞菌的生长呈负相关在高营养水平下。与光合作用和氨基酸合成有关的过表达蛋白质是造成铜绿假单胞菌养分供应增加的刺激作用。氮供应的增加通过诱导细胞分裂调节蛋白FtsZ的过表达而直接促进了蓝细菌的生长。NtcA调节与氮同化和微囊藻毒素合成相关的基因转录，在高氮条件下过表达，因此诱导2种微囊藻毒素合成酶（McyC和McyF）的过表达并促进微囊藻毒素的合成。氮供应增加导致参与气体囊泡组织的蛋白质（GvpC和GvpW）的过表达，这可能会增加铜绿假单胞菌的浮力。磷水平的升高通过介导与碳和磷代谢有关的差异表达蛋白间接影响铜绿假单胞菌的生长和细胞物质的合成。这项研究提供了一种针对铜绿假单胞菌蛋白质组变化对2种关键营养素供应增加的反应的全面描述。