The current study investigated the effects of gamma radiation on the death rate, morphological traits and meiotic abnormalities in ground-grown chrysanthemum ‘Donglinruixue’, and inter-simple sequence repeat (ISSR) markers were used to identify the DNA polymorphism among mutants. The results showed that the death rate significantly increased with increase in radiation dose. Semi-lethal (LD50) dose was approximately 35 Gy. Compared with unirradiated control, plant growth was significantly inhibited. After irradiation, a series of morphological variations and cytological aberrations occurred in radiated plants. The peak in variation frequency appeared at 35 Gy. In total, ISSR analysis produced 72 scorable bands, of which 64 (88.89%) were polymorphic. The current study demonstrated that gamma irradiation generates a sufficient number of induced mutations and that ISSR analysis offered a useful molecular marker analysis for the identification of mutants.

中文翻译：

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菊花“东林瑞雪”中γ射线诱导的形态，细胞学和分子变异

目前的研究调查了γ射线对菊花“东林瑞雪”的死亡率，形态特征和减数分裂异常的影响，并使用简单序列重复（ISSR）标记鉴定了突变体之间的DNA多态性。结果表明，死亡率随着辐射剂量的增加而显着增加。半致死剂量（LD50）约为35 Gy。与未辐照对照相比，植物的生长受到显着抑制。辐照后，辐照植物发生一系列形态学变化和细胞学畸变。变化频率的峰值出现在35 Gy。总的来说，ISSR分析产生了72条可评分带，其中64条（88.89％）是多态的。