Grass pea (Lathyrus sativus L.) is an important food crop cultivated in dryland agricultural ecosystem. It is an important source of dietary protein to millions of people living in low-income countries in South-east Asia and Africa. The present study emphasises the development of genomic resources and their application in marker–trait association for plant phenology and yield-related traits in lathyrus. In silico mining of nucleotide sequences identified 203 simple sequence repeat (SSR) motifs, of which trimer repeats (62%) were most abundant followed by tetramer (19%), hexamer (10%), pentamer (6%) and dimer (3%) nucleotide repeats. Of 150 SSR markers screened, 60 markers were amplified 75 alleles from 50 germplasm lines with 2–3 alleles per locus and the polymorphic information content of 0.45 was observed. We report 6 significant marker–trait associations using the developed SSR markers for plant phenology and yield-related traits following mixed linear model (Q+K) analysis. Gene ontology search of trait linked markers revealed marker regions encoding genes related to homeobox-leucine zipper protein ATHB-6-like, rubredoxin family protein, and cationic peroxidise. Understanding the association of novel alleles in trait expression will play a significant role in future lathyrus crop improvement programmes.

草豌豆（山thy豆）是在旱地农业生态系统中种植的重要粮食作物。它是生活在东南亚和非洲低收入国家的数百万人饮食蛋白质的重要​​来源。本研究强调基因组资源的开发及其在山la豆植物物候和产量相关性状的标记－性状关联中的应用。电脑挖掘核苷酸序列可确定203个简单序列重复（SSR）基序，其中三聚体重复（62％）最丰富，其次是四聚体（19％），六聚体（10％），五聚体（6％）和二聚体（3％）核苷酸重复。在筛选的150个SSR标记中，从50个种质系中扩增了75个等位基因，每个位点为2-3个等位基因，观察到多态信息含量为0.45。我们使用混合线性模型（Q + K）分析后，使用已开发的SSR标记对植物物候和产量相关性状报告了6个重要的标记-性状关联。性状连锁标记的基因本体搜索揭示了标记区域，这些标记区域编码与同源异型盒-亮氨酸拉链蛋白ATHB-6-like，红氧还蛋白家族蛋白和阳离子过氧化物有关的基因。