In an approach combining microbiological culture methods with high-throughput sequencing, this study investigated the microbial communities (bacteria, moulds and yeasts) in Swedish-produced edible crickets (Acheta domesticus) reared in a controlled environment. The effects of different feeds on microbial loads and populations in crickets were also studied. The crickets used were third-generation offspring from wild-caught individuals from Sweden, which are adapted to grow in a laboratory environment. The efficiency of rinsing to decrease microbial load was evaluated not obtaining a significant decrease of plating counts for total aerobic counts (TAC) and Enterobacteriaceae. Crickets were divided into three batches and fed different diets (control feed, early-cut red clover hay (ECH), late-cut fresh red clover (LCF)) for 62 days. Bacterial numbers (TAC and Enterobacteriaceae) on whole raw crickets ranged between 7 and 8 log cfu/g. Pre-rinsing in water did not reduce these levels (P=0.19). All batches tested negative for the food-borne bacteria Salmonella, Listeria monocytogenes, Bacillus cereus and Clostridium perfringens. The mean mould count for crickets fed control feed was 2.8 log cfu/g, while the values for crickets fed ECH and LCF were 4.2 and 4.5 log cfu/g, respectively. The dominant bacterial communities were Proteobacteria, Bacteroidetes and Firmicutes, with Firmicutes and Proteobacteria dominating in crickets fed control feed, Firmicutes dominating in crickets fed LCF and Proteobacteria dominating in crickets fed ECH. Aspergillus flavus, a fungus that is capable of producing mycotoxins, was detected in control feed and ECH reared crickets. More work is needed to identify specific food-borne pathogens in edible crickets and establish possible bacterial quality reference values, as an important step in developing microbial quality and safety parameters to ensure consumer safety.

中文翻译：

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controlled（Acheta domesticus）的微生物群落和食品安全方面的控制条件

通过将微生物培养方法与高通量测序相结合的方法，本研究调查了瑞典生产的edible（Acheta domesticus）中的微生物群落（细菌，霉菌和酵母菌））在受控环境中饲养。还研究了不同饲料对微生物负荷和种群的影响。used是来自瑞典的野生捕获个体的第三代后代，适合在实验室环境中生长。对于总有氧计数（TAC）和肠杆菌科，未评估漂洗降低微生物负荷的效率，但未发现接种计数的显着降低。分为三批，分别饲喂62天的不同日粮（对照饲料，早切红三叶草干草（ECH），晚切鲜红三叶草（LCF））。整个生的细菌数量（TAC和肠杆菌科）介于7和8 log cfu / g之间。在水中预先冲洗并没有降低这些水平（P= 0.19）。所有批次的食源细菌沙门氏菌，单核细胞增生性李斯特菌，蜡样芽孢杆菌和产气荚膜梭状芽胞杆菌均呈阴性。control饲喂对照饲料的mold平均霉菌数为2.8 log cfu / g，而E饲ECH和LCF喂饲的霉菌值分别为4.2和4.5 log cfu / g。优势细菌群落为Proteobacteria，Bacteroidetes和Firmicutes，其中以ic菌和Proteobacteria为主的control饲喂对照饲料，以Firmicutes为主的L饲LCF饲料和以Proteobacteria为主的E饲ECH。黄曲霉对照饲料和ECH饲养的cket中检出了一种能够产生霉菌毒素的真菌。作为确定微生物质量和安全性参数以确保消费者安全的重要步骤，需要做更多的工作来识别可食用中特定的食源性病原体，并确定可能的细菌质量参考值。