Abstract. Lensless inline digital holographic microscopy (LI-DHM) and Fourier ptychographic microscopy (FPM) are two widespread quantitative phase imaging (QPI) techniques. They have been employed in various fields, especially for biological slice imaging because of their simplicity in use, stability in structure, and also large field of view. Spherical phase response (for example from HeLa cells) is commonly observed in biological imagery. As a consequence, for calibration and validation purposes, small (several to tenth of microns in diameter) transparent microbeads have been used as standards. Phase imaging of their large counterparts (hundreds of microns in diameter) using either LI-DHM or FPM has not been reported so far. We are aiming to analyze the phase response of a 146-μm soda-lime microsphere. It has been immersed in Canada balsam to reduce phase difference and to avoid overexposed diffraction rings. The phase estimation issue has been tackled using approaches that involve either Gerchberg–Saxton type algorithms or an inverse problem-based procedure. Confronting the results confirms the QPI capability for both imaging techniques to assess phase responses from such a large transparent object.

中文翻译：

---

无透镜在线数字全息与傅立叶 ptychography：大透明珠的相位估计

摘要。无透镜在线数字全息显微镜 (LI-DHM) 和傅立叶 ptychographic 显微镜 (FPM) 是两种广泛使用的定量相位成像 (QPI) 技术。由于其使用简单、结构稳定、视野大，它们已被应用于各个领域，特别是生物切片成像。球形相位响应（例如来自 HeLa 细胞）通常在生物图像中观察到。因此，出于校准和验证的目的，小（直径为几微米到十分之一微米）的透明微珠已被用作标准。迄今为止，尚未报道使用 LI-DHM 或 FPM 对其大型对应物（直径数百微米）进行相位成像。我们的目标是分析 146 微米钠钙微球的相位响应。它已浸入加拿大香脂中以减少相位差并避免过度曝光的衍射环。已经使用涉及 Gerchberg-Saxton 类型算法或基于逆问题的程序的方法解决了相位估计问题。面对结果证实了两种成像技术的 QPI 能力，可以评估来自如此大的透明物体的相位响应。