Abstract. Tumor necrosis factor-alpha (TNF-α) is a signaling protein of inflammatory processes. TNF-α overexpression triggers inflammatory processes related to various diseases such as rheumatoid arthritis, Crohn’s disease, Chagas, and others. For this reason, the TNF-α has been used as an important biological biomarker for prognosis, understanding, and disease treatment. Surface plasmon resonance (SPR)-based biosensors are a good alternative for TNF-α detection because they are more sensitive than other techniques and have the ability to be developed miniaturized systems. Then we developed a nanohole array on gold nanofilm-based SPR biosensor for TNF-α detection. A biological biorecognition system (cysteamine/biotin/streptavidin/TNF-α antibody) was formed and TNF-α antigen was detected by transmitted light intensity monitoring. TNF-α antigen at 17 pg mL − 1 was detected from fungus Paracoccidioides brasiliensis-infected rat blood serum. This concentration is far below those found in similar studies in the literature. This plasmonic device opens new opportunities for TNF-α detection.

中文翻译：

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基于金纳米孔阵列的等离子体生物传感器检测 Pb18 感染大鼠血清中肿瘤坏死因子-α 细胞因子

摘要。肿瘤坏死因子-α (TNF-α) 是炎症过程的信号蛋白。TNF-α 过度表达会触发与各种疾病相关的炎症过程，例如类风湿性关节炎、克罗恩病、恰加斯病等。因此，TNF-α 已被用作重要的生物标志物，用于预后、理解和疾病治疗。基于表面等离子体共振 (SPR) 的生物传感器是 TNF-α 检测的一个很好的替代方案，因为它们比其他技术更敏感，并且有能力开发小型化系统。然后我们在基于金纳米膜的 SPR 生物传感器上开发了一个纳米孔阵列，用于 TNF-α 检测。形成生物生物识别系统（半胱胺/生物素/链霉亲和素/TNF-α抗体），通过透射光强度监测检测TNF-α抗原。从真菌 Paracoccidioides brasiliensis 感染的大鼠血清中检测到 17 pg mL - 1 的 TNF-α 抗原。该浓度远低于文献中类似研究中发现的浓度。这种等离子体装置为 TNF-α 检测开辟了新的机会。