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Association of miR-196a2 and miR-149 single-nucleotide polymorphisms with atherosclerotic ischemic stroke susceptibility
The Egyptian Journal of Neurology, Psychiatry and Neurosurgery ( IF 1.1 ) Pub Date : 2020-08-20 , DOI: 10.1186/s41983-020-00219-7
Dina Mahmoud , Ola El-Sisi , Marwa Sheta , Sandra Ahmed , Mona Fathy , Kareeman Gomaa , Tarek Ramzy

Background Beside common risk factors for stroke such as diabetes and hypertension, single-nucleotide variants occurring within micro RNA genes have been identified as susceptibility loci for ischemic stroke risk. Objectives Investigate the possible association of two variants in pre miRNA sequences, rs11614913 within miR-196a2 C > T and rs2292832 within miR-149 T > C, with ischemic stroke. Subjects and methods One hundred ischemic stroke patients and 100 age and sex-matched controls having > 1 risk factor for atherosclerosis were enrolled in a case-control study. Degree of atherosclerosis was assessed using ultrasonography. Micro RNA variants were assessed by real-time PCR TaqMan probe assay. Results The TT genotype and T allele frequencies of miR-196a2 C > T were protective against ischemic stroke (OR 0.168, P 0.001; OR 0.482, P < 0.001 respectively). While among miR-149 T > C variants, CC genotype was associated with increased risk by threefold (OR 3.061, P 0.005) and C allele was associated with about 1.9 fold risk of stroke (OR 1.909, P 0.002). Haplotypes analysis revealed miR-196a2T/-149 T allele combination was significantly lower among stroke patients than the controls ( P < 0.001) with a protective effect (OR 0.196, 95% CI 0.083-0.466). Conclusion A decrease in the incidence of ischemic stroke is associated with miR-196a2 TT genotype and T allele and increases in the likelihood risk of ischemic stroke are associated with CC genotype and C allele within miR-149, and the two miRNAs under study are closely associated with vascular damage responses.

中文翻译:

miR-196a2和miR-149单核苷酸多态性与动脉粥样硬化缺血性卒中易感性的相关性

背景 除了糖尿病和高血压等中风的常见危险因素外,微小 RNA 基因内发生的单核苷酸变异已被确定为缺血性中风风险的易感位点。目的 研究 pre miRNA 序列中的两个变体,miR-196a2 C > T 中的 rs11614913 和 miR-149 T > C 中的 rs2292832 与缺血性中风的可能关联。受试者和方法 在病例对照研究中招募了 100 名缺血性中风患者和 100 名年龄和性别匹配的具有 > 1 个动脉粥样硬化危险因素的对照。使用超声检查评估动脉粥样硬化的程度。Micro RNA 变体通过实时 PCR TaqMan 探针测定进行评估。结果 miR-196a2 C > T 的 TT 基因型和 T 等位基因频率对缺血性卒中具有保护作用(OR 0.168,P 0.001;OR 0.482,P < 0.001)。而在 miR-149 T > C 变体中,CC 基因型与增加三倍的风险相关(OR 3.061,P 0.005),而 C 等位基因与约 1.9 倍的中风风险相关(OR 1.909,P 0.002)。单倍型分析显示,卒中患者的 miR-196a2T/-149 T 等位基因组合显着低于对照组(P < 0.001),具有保护作用(OR 0.196,95% CI 0.083-0.466)。结论 缺血性卒中发生率的降低与 miR-196a2 TT 基因型和 T 等位基因相关,缺血性卒中可能性的增加与 miR-149 内的 CC 基因型和 C 等位基因相关,研究中的两种 miRNA 密切相关。与血管损伤反应有关。CC 基因型与风险增加三倍相关(OR 3.061,P 0.005),C 等位基因与卒中风险增加约 1.9 倍(OR 1.909,P 0.002)相关。单倍型分析显示,卒中患者的 miR-196a2T/-149 T 等位基因组合显着低于对照组(P < 0.001),具有保护作用(OR 0.196,95% CI 0.083-0.466)。结论 缺血性卒中发生率的降低与 miR-196a2 TT 基因型和 T 等位基因相关,缺血性卒中可能性的增加与 miR-149 内的 CC 基因型和 C 等位基因相关,研究中的两种 miRNA 密切相关。与血管损伤反应有关。CC 基因型与风险增加三倍相关(OR 3.061,P 0.005),C 等位基因与卒中风险增加约 1.9 倍(OR 1.909,P 0.002)相关。单倍型分析显示,卒中患者的 miR-196a2T/-149 T 等位基因组合显着低于对照组(P < 0.001),具有保护作用(OR 0.196,95% CI 0.083-0.466)。结论 缺血性卒中发生率的降低与 miR-196a2 TT 基因型和 T 等位基因相关,缺血性卒中可能性的增加与 miR-149 内的 CC 基因型和 C 等位基因相关,研究中的两种 miRNA 密切相关。与血管损伤反应有关。单倍型分析显示,卒中患者的 miR-196a2T/-149 T 等位基因组合显着低于对照组(P < 0.001),具有保护作用(OR 0.196,95% CI 0.083-0.466)。结论 缺血性卒中发生率的降低与 miR-196a2 TT 基因型和 T 等位基因相关,缺血性卒中可能性的增加与 miR-149 内的 CC 基因型和 C 等位基因相关,研究中的两种 miRNA 密切相关。与血管损伤反应有关。单倍型分析显示,卒中患者的 miR-196a2T/-149 T 等位基因组合显着低于对照组(P < 0.001),具有保护作用(OR 0.196,95% CI 0.083-0.466)。结论 缺血性卒中发生率的降低与 miR-196a2 TT 基因型和 T 等位基因相关,缺血性卒中可能性的增加与 miR-149 内的 CC 基因型和 C 等位基因相关,研究中的两种 miRNA 密切相关。与血管损伤反应有关。
更新日期:2020-08-20
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