Hydrogen sulphide (H2S) is involved in regulating physiological processes in plants. We investigated how H2S ameliorates iron (Fe) deficiency in soybean (Glycine max L.) seedlings. Multidisciplinary approaches including physiological, biochemical and molecular, and transcriptome methods were used to investigate the H2S role in regulating Fe availability in soybean seedlings. Our results showed that H2S completely prevented leaf interveinal chlorosis and caused an increase in soybean seedling biomass under Fe deficiency conditions. Moreover, H2S decreased the amount of root-bound apoplastic Fe and increased the Fe content in leaves and roots by regulating the ferric-chelate reductase (FCR) activities and Fe homeostasis- and sulphur metabolism-related gene expression levels, thereby promoting photosynthesis in soybean seedlings. In addition, H2S changed the plant hormone concentrations by modulating plant hormone-related gene expression abundances in soybean seedlings grown in Fe-deficient solution. Furthermore, organic acid biosynthesis and related genes expression also played a vital role in modulating the H2S-mediated alleviation of Fe deficiency in soybean seedlings. Our results indicated that Fe deficiency was alleviated by H2S through enhancement of Fe acquisition and assimilation, thereby regulating plant hormones and organic acid synthesis in plants.

中文翻译：

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硫化氢通过促进大豆幼苗的铁利用率和植物激素水平来缓解铁缺乏症。


硫化氢 (H2S) 参与调节植物的生理过程。我们研究了 H2S 如何改善大豆 (Glycine max L.) 幼苗的铁 (Fe) 缺乏。采用生理、生化、分子和转录组等多学科方法研究 H2S 在调节大豆幼苗铁利用率中的作用。我们的结果表明，在缺铁条件下，H2S 完全防止了叶脉间失绿，并导致大豆幼苗生物量增加。此外，H2S通过调节铁螯合还原酶（FCR）活性以及铁稳态和硫代谢相关基因的表达水平，降低了根系质外体铁的含量，增加了叶和根中铁的含量，从而促进了大豆的光合作用幼苗。此外，H2S 通过调节缺铁溶液中生长的大豆幼苗中植物激素相关基因的表达丰度来改变植物激素浓度。此外，有机酸生物合成和相关基因表达在调节H2S介导的缓解大豆幼苗铁缺乏方面也发挥着重要作用。我们的研究结果表明，H2S 通过增强铁的吸收和同化来缓解铁缺乏，从而调节植物激素和有机酸的合成。