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Chitosan-polydopamine hydrogel complex: a novel green adhesion agent for reversibly bonding thermoplastic microdevice and its application for cell-friendly microfluidic 3D cell culture.
Lab on a Chip ( IF 6.1 ) Pub Date : 2020-08-20 , DOI: 10.1039/d0lc00621a
Kieu The Loan Trinh 1 , Nguyen Xuan Thanh Le , Nae Yoon Lee
Affiliation  

Owing to biocompatible characteristics and supporting cell growth capability, hydrogels have been widely used for scaffold fabrication and surface coating for cell culture. To employ the advantages of hydrogels, in the present study, we introduce a biocompatible chitosan (CS)–polydopamine (pDA) hydrogel complex as a green adhesion agent for the reversible bonding of thermoplastics assisted by UV irradiation. Poly(methyl methacrylate) (PMMA) substrates were bonded due to the covalent bond network formed between the amine groups of either CS or pDA in the hydrogel complex and the aldehyde groups of the oxidized PMMA surface via the Schiff-base reaction during the UV irradiation. Furthermore, the introduced method allowed for reversible bonding, which is highly appropriate for the fabrication of microdevices for cell-related applications. Surface characterizations such as water contact angle measurement, scanning electron microscopy analysis (SEM), atomic force microscopy analysis (AFM), and Fourier-transform infrared microscopy analysis (FTIR) were performed to confirm the successful coating of the hydrogel complex on the PMMA surface. Moreover, the bonding between two PMMAs or PMMA with other thermoplastics was successfully investigated with high bond strengths ranging from 0.4 to 0.7 MPa. The potential for reversible bonding of this method was verified by repeating the bonding/debonding cycle of the bonded PMMAs for three times, which maintained the bond strength at approximately 0.5 MPa. The compatibility of the bonding method in biological applications was examined by culturing mesenchymal stem cells (MSCs) inside a microchannel where multiple uniform-sized MSC spheroids were successfully formed. Then, spheroids were harvested for off-chip experiments enabled by the reversibility of the introduced bonding strategy. The bonding strategy employing a green hydrogel complex as a cell-friendly and eco-friendly adhesion agent could have a high impact on the fabrication of microdevices suitable for advanced organ-on-a-chip studies.

中文翻译:

壳聚糖-聚多巴胺水凝胶复合物:一种可逆地粘合热塑性微器件的新型绿色黏合剂及其在对细胞友好的微流体3D细胞培养中的应用。

由于具有生物相容性和支持细胞生长的能力,水凝胶已被广泛用于支架制造和细胞培养的表面涂层。为了利用水凝胶的优势,在本研究中,我们引入了生物相容性壳聚糖(CS)-聚多巴胺(pDA)水凝胶复合物,作为绿色胶粘剂,用于通过紫外线照射辅助热塑性塑料的可逆粘合。由于在水凝胶复合物中CS或pDA的胺基和氧化的PMMA表面的醛基之间形成了共价键网络,因此粘合了聚甲基丙烯酸甲酯(PMMA)基材在紫外线照射下的席夫碱反应。此外,引入的方法允许可逆键合,这非常适合于制造与细胞相关的应用的微器件。进行了表面表征,例如水接触角测量,扫描电子显微镜分析(SEM),原子力显微镜分析(AFM)和傅里叶变换红外显微镜分析(FTIR),以确认水凝胶复合物在PMMA表面上的成功涂层。此外,成功地研究了两种PMMA或PMMA与其他热塑性塑料之间的粘结,粘结强度范围为0.4至0.7 MPa。通过重复三次键合的PMMA的键合/脱键循环,验证了该方法可逆键合的潜力,其将粘结强度保持在约0.5 MPa。通过在成功形成多个均匀大小的M​​SC球体的微通道内培养间充质干细胞(MSC),检查了结合方法在生物应用中的兼容性。然后,通过引入的键合策略的可逆性,收集球状体用于芯片外实验。采用绿色水凝胶复合物作为对细胞友好和对环境友好的黏合剂的结合策略可能会对适合进行高级器官上芯片研究的微器件的制造产生重大影响。通过引入的键合策略的可逆性,可以收集球状体用于芯片外实验。采用绿色水凝胶复合物作为对细胞友好和对环境友好的黏合剂的结合策略可能会对适合进行高级器官上芯片研究的微器件的制造产生重大影响。通过引入的键合策略的可逆性,可以将球状体收获用于芯片外实验。采用绿色水凝胶复合物作为对细胞友好和对环境友好的黏合剂的结合策略可能会对适合于高级芯片上器官研究的微器件的制造产生重大影响。
更新日期:2020-09-29
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