Fetal bovine serum (FBS) used in cell culture may be contaminated with adventitious agents, which can affect the production of biologicals and the results of clinical laboratory tests. We carried out a retrospective study to determine the incidence of adventitious agent contamination of Argentinean irradiated FBS dating from 2015 to 2019. We analyzed FBS batches for mycoplasma and adventitious viruses (bovine pestiviruses, bovine adenovirus, bluetongue virus, bovine parainfluenza virus 3, rabies virus, bovine parvovirus, bovine herpesvirus 1, bovine respiratory syncytial virus, and reovirus). Cell passages followed by direct immunofluorescence were carried out to check viability of the mentioned adventitious agents. Also, molecular detection of mycoplasma and pestiviruses was performed on the FBS samples. The presence of neutralizing antibodies against pestiviruses was determined. Molecular analyses indicated that frequencies of mycoplasma and pestiviruses in FBS were 14% and 84%, respectively. All of the batches were seronegative for pestiviral antibodies. After cell passages, all FBS samples were negative for hemadsorbent agents and by immunofluorescence for all of the viral species analyzed; PCR assays were negative for mycoplasma and pestiviruses. Our results demonstrate that, of all adventitious agents tested, local FBS batches only had traces of mycoplasma and pestiviruses; gamma irradiation was effective in inactivating them.

细胞培养中使用的胎牛血清 (FBS) 可能被外源物质污染，这会影响生物制品的生产和临床实验室测试的结果。我们进行了一项回顾性研究，以确定 2015 年至 2019 年阿根廷辐照 FBS 外源因子污染的发生率。我们分析了 FBS 批次的支原体和外源病毒（牛瘟病毒、牛腺病毒、蓝舌病毒、牛副流感病毒 3、狂犬病病毒） 、牛细小病毒、牛疱疹病毒 1、牛呼吸道合胞病毒和呼肠孤病毒）。进行细胞传代，然后进行直接免疫荧光检测以检查提到的外来因子的活力。此外，对 FBS 样品进行了支原体和瘟病毒的分子检测。确定了针对瘟病毒的中和抗体的存在。分子分析表明，FBS 中支原体和瘟病毒的频率分别为 14% 和 84%。所有批次的瘟病毒抗体均为血清阴性。细胞传代后，所有 FBS 样品的血液吸附剂和所有分析的病毒种类的免疫荧光均呈阴性；PCR 检测对支原体和瘟病毒呈阴性。我们的结果表明，在所有测试的外来因子中，本地 FBS 批次只有支原体和瘟病毒的痕迹；伽马辐射能有效地灭活它们。细胞传代后，所有 FBS 样品的血液吸附剂和所有分析的病毒种类的免疫荧光均呈阴性；PCR 检测对支原体和瘟病毒呈阴性。我们的结果表明，在所有测试的外来因子中，本地 FBS 批次只有支原体和瘟病毒的痕迹；伽马辐射能有效地灭活它们。细胞传代后，所有 FBS 样品的血液吸附剂和所有分析的病毒种类的免疫荧光均呈阴性；PCR 检测对支原体和瘟病毒呈阴性。我们的结果表明，在所有测试的外来因子中，本地 FBS 批次只有支原体和瘟病毒的痕迹；伽马辐射能有效地灭活它们。