Nonylphenol (NP) is an environmental contaminant, which adversely affects the male fertility due to endocrine disruption and generation of oxidative stress. The current research was planned to assess the effects of nobiletin (NOB), a polymethoxyflavone, on NP-induced testicular damages. Twenty-four male rats were divided into 4 groups: control (0.1% DMSo), NP group (50 mg/kg), NP+NOB group (50 mg/kg + 25 mg/kg), and NOB group (25 mg/kg). Our results revealed that NP brought down the activities of catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GPx) and glutathione reductase (GSR), while elevated the level of thiobarbituric acid reactive substances (TBARS). Additionally, NP decreased the level of follicle-stimulating hormone (FSH), luteinizing hormone (LH), plasma testosterone, daily sperm production (DSP), epididymal sperm count, viability, motility, gene expression of testicular steroidogenic enzymes (StAR, 3β-HSD and 17β-HSD) and anti-apoptotic protein (Bcl-2), as well as number of spermatogenic cells belonging to various stages. Whereas, sperm (head, mid-piece/neck and tail) abnormalities, expression of apoptotic proteins (Bax and caspase-3), and histopathological damages were increased. However, NOB remarkably reversed all the damages caused by NP. Therefore, it is deduced that NOB could be used as a potential therapeutic to counter the NP-prompted oxidative stress and apoptotic damages in testes.

壬基酚 (NP) 是一种环境污染物，会因内分泌干扰和氧化应激的产生而对男性生育能力产生不利影响。目前的研究计划评估川陈皮素 (NOB)（一种多甲氧基黄酮）对 NP 引起的睾丸损伤的影响。将 24 只雄性大鼠分为 4 组：对照组（0.1% DMSo）、NP 组（50 mg/kg）、NP+NOB 组（50 mg/kg + 25 mg/kg）、NOB 组（25 mg/kg）。千克）。我们的结果显示，NP降低了过氧化氢酶（CAT）、超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GPx）和谷胱甘肽还原酶（GSR）的活性，同时升高了硫代巴比妥酸反应物质（TBARS）的水平。此外，NP 还可降低卵泡刺激素 (FSH)、黄体生成素 (LH)、血浆睾酮、每日精子生成量 (DSP)、附睾精子计数、活力、运动性、睾丸类固醇生成酶（StAR、3β- HSD 和 17β-HSD）和抗凋亡蛋白（Bcl-2），以及属于各个阶段的生精细胞的数量。然而，精子（头部、中段/颈部和尾部）异常、凋亡蛋白（Bax 和 caspase-3）表达以及组织病理学损伤增加。然而，NOB 显着扭转了 NP 造成的所有损害。因此，推测NOB可以作为一种潜在的治疗方法来对抗NP引起的睾丸氧化应激和细胞凋亡损伤。