Lycopene (lyc) has an effect on preventing cancer, yet its effects on hypoxia/reoxygenation (H/R) injury remained obscure. The study aimed at discovering its role in preventing hepatic cells against H/R injury. Hepatic cells were incubated in hypoxia incubator to simulate ischemia/reperfusion injury in vitro. Cell viability was detected using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay after Lycopene treatment with or without ML385 (nuclear factor erythroid 2-related factor 2 [Nrf2] inhibitor). Lactate dehydrogenase (LDH) and malondialdehyde (MDA) content were detected. Cellular cytokine (tumor necrosis factor-α, TNF-α; interleukin-6, IL-6) levels were measured using enzyme-linked immunosorbent assay (ELISA). Hepatic cell apoptosis and cellular reactive oxygen species (ROS) content was detected by flow cytometry. Nrf2 transfer was observed using immunofluorescence staining. Nrf2 and heme oxygenase-1 (HO-1) expressions were detected with quantitative real-time polymerase chain reaction and western blot as needed. In hepatic cells, after H/R, the viability was dropped, TNF-α and IL-6 levels and LDH and MDA content were increased, with high apoptosis rate and ROS content. Lycopene led to a reversed effect, with promotion on Nrf2 transfer from cytoplasm into nucleus and Nrf2/HO-1 pathway activation. Further experiments showed that ML385 could reverse the effects of Lycopene. Lycopene could activate Nrf2/HO-1 pathway to protect hepatic cells against H/R injury.

中文翻译：

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番茄红素通过 AML12 细胞中的 Nrf2/HO-1 通路减轻肝缺氧/复氧损伤。

番茄红素 (lyc) 具有预防癌症的作用，但其对缺氧/复氧 (H/R) 损伤的影响仍不清楚。该研究旨在发现其在预防肝细胞对抗 H/R 损伤中的作用。肝细胞在缺氧培养箱中培养以模拟体外缺血/再灌注损伤. 在使用或不使用 ML385（核因子红细胞 2 相关因子 2 [Nrf2] 抑制剂）处理番茄红素后，使用 3-(4,5-二甲基噻唑-2-基)-2,5-二苯基溴化四唑 (MTT) 测定法检测细胞活力. 检测乳酸脱氢酶（LDH）和丙二醛（MDA）含量。使用酶联免疫吸附试验 (ELISA) 测量细胞细胞因子（肿瘤坏死因子-α、TNF-α；白细胞介素-6、IL-6）水平。通过流式细胞术检测肝细胞凋亡和细胞活性氧（ROS）含量。使用免疫荧光染色观察 Nrf2 转移。根据需要，通过定量实时聚合酶链反应和蛋白质印迹检测 Nrf2 和血红素加氧酶-1 (HO-1) 的表达。在肝细胞中，H/R 后，活力下降，TNF-α 和 IL-6 水平以及 LDH 和 MDA 含量增加，具有高凋亡率和 ROS 含量。番茄红素导致逆转作用，促进 Nrf2 从细胞质转移到细胞核和 Nrf2/HO-1 通路激活。进一步的实验表明，ML385 可以逆转番茄红素的作用。番茄红素可以激活 Nrf2/HO-1 通路保护肝细胞免受 H/R 损伤。