In this study, we studied the DNA extraction capability of poly(4,4-cyclohexylidene bisphenol oxalate) following the surface modification and composite formation with that of microcrystalline cellulose (MCC) and magnetic iron oxide nanoparticles (NPs). The physical characterization techniques like scanning electron microscopy (SEM), Fourier-transform infrared (FTIR) spectroscopy, energy-dispersive X-ray analysis (EDX), and thermogravimetric analysis (TGA) were employed for the poly(bisphenol Z oxalate)-MCC-magnetite composite during different stages of its formation. The results confirmed the successful modification of the polymer surface. On testing in the presence of three types of binding buffers, a high value of 72.4% (out of 10,000 ng/μL) efficiency with a total yield of DNA at ng and absorbance ratio of A260/A280 (1.980) was observed for the 2 M GuHCl/EtOH binding buffer. These results were compared against the other two buffers of phosphate-buffered saline (PBS) and NaCl. The lowest value of DNA extraction efficiency at 8125 ng/μL of 58.845% with absorbance ratios of A260/A280 (1.818) for PBS was also observed. The study has concluded an enhancement in the DNA extraction efficiency when the polymer is in the composite stage along with cellulose and magnetite particles as compared against the bare polymer.

中文翻译：

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聚（4,4-亚环己基双酚草酸酯）修饰的微晶纤维素-磁铁矿复合材料的高效DNA提取

在这项研究中，我们研究了聚DNA提取能力（4,4 -以下的表面改性和复合材料的形成与微晶纤维素（MCC）和磁性氧化铁纳米颗粒（NP）的亚环己基双酚草酸盐）。聚（草酸双酚Z）-MCC采用了诸如扫描电子显微镜（SEM），傅立叶变换红外（FTIR）光谱，能量色散X射线分析（EDX）和热重分析（TGA）的物理表征技术-磁铁矿复合物在其形成的不同阶段。结果证实了聚合物表面的成功改性。对三种类型的结合缓冲液，72.4％的高值的存在检测（下10,000纳克/ μ L）效率与DNA的总产对于2 M GuHCl / EtOH结合缓冲液，观察到ng和A260 / A280的吸光度比（1.980）。将这些结果与磷酸盐缓冲盐水（PBS）和NaCl的其他两种缓冲液进行了比较。的DNA提取效率在8125纳克/最低值μ与A260 / A280（1.818）对PBS吸光度比L的58.845％，也观察到。研究得出结论，与裸露的聚合物相比，当聚合物与纤维素和磁铁矿颗粒处于复合阶段时，DNA提取效率得到了提高。