Allying high-resolution with a large field-of-view (FOV) is of great importance in the fields of biology and medicine, but it is particularly challenging when imaging non-flat living samples such as the human retina. Indeed, high-resolution is normally achieved with adaptive optics (AO) and scanning methods, which considerably reduce the useful FOV and increase the system complexity. An alternative technique is time-domain full-field optical coherence tomography (FF-OCT), which has already shown its potential for in-vivo high-resolution retinal imaging. Here, we introduce coherence gate shaping for FF-OCT, to optically shape the coherence gate geometry to match the sample curvature, thus achieving a larger FOV than previously possible. Using this instrument, we obtained high-resolution images of living human photoreceptors close to the foveal center without AO and with a 1 mm × 1 mm FOV in a single shot. This novel advance enables the extraction of photoreceptor-based biomarkers with ease and spatiotemporal monitoring of individual photoreceptors. We compare our findings with AO-assisted ophthalmoscopes, highlighting the potential of FF-OCT, as a compact system, to become a routine clinical imaging technique.

中文翻译：

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相干门整形通过全场OCT进行大视野高分辨率体内视网膜成像

在生物学和医学领域中，将高分辨率与大视场（FOV）结合在一起非常重要，但是在对诸如人体视网膜之类的非平坦生物样品成像时，这尤其具有挑战性。实际上，高分辨率通常是通过自适应光学（AO）和扫描方法实现的，这大大降低了有用的FOV并增加了系统复杂性。另一种替代技术是时域全场光学相干断层扫描（FF-OCT），该技术已经显示出其在体内的潜力高分辨率视网膜成像。在这里，我们介绍了用于FF-OCT的相干门整形，以光学方式对相干门几何形状进行整形以匹配样本曲率，从而实现了比以前更大的FOV。使用该仪器，我们获得了靠近中央凹中心的活人感光体的高分辨率图像，其中没有AO，单次拍摄的FOV为1 mm ×1 mm。这一新颖的进展使得能够轻松且时空监控单个感光体来提取基于感光体的生物标记。我们将我们的发现与AO辅助检眼镜进行比较，强调了FF-OCT作为一种紧凑系统的潜力，成为常规的临床成像技术。