Introduction
Glioma is a common type of neoplasm that occurs in the central nervous system. miRNAs have been demonstrated to act as critical regulators of carcinogenesis and tumor progression in multiple cancers, but the molecular mechanism of miR-330-3p in glioma remained unclear. The purpose of the study was to explore the role of miR-330-3p in glioma cell reproduction and migration.

Material and methods
The expression levels of miR-330-3p and CELF1 in 27 glioma tissue specimens and human glioma cell lines were examined by qRT-PCR and western blot. The TargetScan database was used to predict the relationship between miR-330-3p and CELF1. Then the target relationship was verified using dual-luciferase reporter assay. The effects of miR-330-3p/CELF1 on glioma cell proliferation were evaluated by MTT and colony formation assay. Wound healing assay was employed to measure the migration ability of glioma cells.

Results
MiR-330-3p was found lowly expressed in glioma tissues and cells compared with adjacent tissues and normal astrocytes, while CELF1 expression was relatively high in the glioma tissues and cells. Dual-luciferase reporter assay confirmed that miR-330-3p could directly target CELF1. Furthermore, miR-330-3p could down-regulate the expression of CELF1, therefore suppressing glioma cell reproduction and migration.

Conclusions
MiR-330-3p inhibited the propagation and migration of glioma cells by repressing CELF1 expression.



中文翻译：

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MiR-330-3p 作为一种肿瘤抑制因子，通过靶向 CELF1 来调节胶质瘤细胞的增殖和迁移。

简介
胶质瘤是一种常见的中枢神经系统肿瘤。已证明 miRNA 在多种癌症中充当癌变和肿瘤进展的关键调节因子，但 miR-330-3p 在胶质瘤中的分子机制仍不清楚。该研究的目的是探索 miR-330-3p 在胶质瘤细胞繁殖和迁移中的作用。

材料与方法
通过 qRT-PCR 和蛋白质印迹检测 27 个胶质瘤组织标本和人胶质瘤细胞系中 miR-330-3p 和 CELF1 的表达水平。TargetScan 数据库用于预测 miR-330-3p 和 CELF1 之间的关系。然后使用双荧光素酶报告基因分析验证目标关系。通过MTT和集落形成测定评估miR-330-3p/CELF1对胶质瘤细胞增殖的影响。伤口愈合试验用于测量神经胶质瘤细胞的迁移能力。

结果
与邻近组织和正常星形胶质细胞相比，MiR-330-3p在胶质瘤组织和细胞中的表达较低，而CELF1在胶质瘤组织和细胞中的表达相对较高。双荧光素酶报告基因检测证实 miR-330-3p 可以直接靶向 CELF1。此外，miR-330-3p 可以下调 CELF1 的表达，从而抑制胶质瘤细胞的繁殖和迁移。

结论
MiR-330-3p通过抑制CELF1的表达来抑制胶质瘤细胞的增殖和迁移。