To investigate the effect of bone marrow mesenchymal stem cells (MSCs) on ovarian and testicular function of aging Sprague-Dawley (SD) rats induced by D-galactose (D-gal) and try to clarify the underlying functional mechanism. Adherent culture was used to isolate and purify rat MSCs. The status, proliferation and differentiation of MSCs were detected by hematoxylin-eosin staining, MTT, colony formation, flow cytometry and directional differentiation. The aging rat model was established by subcutaneous injection of D-gal, and the homing of MSCs was detected by fluorescence microscope after infusion of GFP-labeled MSCs through caudal vein. ELISA was used to detect the content of sex hormone in serum, and HE staining was used to observe the structure and morphology of testis and ovary. The isolated and purified MSCs were in good condition, and most of the cells were in G1 phase, which had strong abilities of cell proliferation, colony formation and differentiation. After GFP-labeled MSCs were infused, MSCs could be homed into the testis and ovary of rats. MSCs infusion could significantly improve the morphology of testis and ovary, increase the contents of P and E2 while decrease the contents of LH and FSH in female rats, and increase the content of testosterone in male rats (P < 0.01). It also increased the activity of superoxide dismutase (SOD) in serum of ovary and testis and significantly decreased the content of malondialdehyde (MDA). MSCs affected the content of MDA and the activity of SOD by reducing the expression of cyclin-dependent kinase inhibitor 2A (p16) and increasing proliferating cell nuclear antigen (PCNA), consequently improving the aging and injury of reproductive organs.

研究骨髓间充质干细胞（MSCs）对D-半乳糖（D-gal）诱导的衰老Sprague-Dawley（SD）大鼠卵巢和睾丸功能的影响，并试图阐明其潜在的功能机制。贴壁培养用于分离和纯化大鼠 MSC。通过苏木精-伊红染色、MTT、集落形成、流式细胞术和定向分化检测MSCs的状态、增殖和分化。通过皮下注射D-gal建立衰老大鼠模型，通过尾静脉注入GFP标记的MSCs后，通过荧光显微镜检测MSCs的归巢。ELISA检测血清性激素含量，HE染色观察睾丸和卵巢的结构和形态。分离纯化的MSCs状态良好，且大部分细胞处于G1期，具有较强的细胞增殖、集落形成和分化能力。输注GFP标记的MSCs后，MSCs可以进入大鼠的睾丸和卵巢。MSCs输注可显着改善雌性大鼠睾丸和卵巢形态，增加P和E2含量，降低雌性大鼠LH和FSH含量，增加雄性大鼠睾酮含量。P < 0.01)。它还增加了卵巢和睾丸血清中超氧化物歧化酶（SOD）的活性，并显着降低了丙二醛（MDA）的含量。间充质干细胞通过降低细胞周期蛋白依赖性激酶抑制剂2A（p16）的表达和增加增殖细胞核抗原（PCNA）的表达来影响MDA的含量和SOD的活性，从而改善生殖器官的衰老和损伤。