Abstract

We investigated the protective effect of green tea on diabetic hepato-renal complications. Thirty male Wistar rats were randomly divided into five equal groups: normal control, diabetic control, glibenclamide-treated, green tea-treated, and combined therapy-treated groups; ethical approval number “BERC-014-01-20.” After eight weeks, animals were sacrificed by CO₂ euthanasia method, liver and kidney tissues were processed and stained for pathological changes, and blood samples were collected for biochemical analysis. Diabetic rats showed multiple hepato-renal morphological and apoptotic changes associated with significantly increased some biochemical parameters, while serum albumin and HDL decreased significantly compared to normal control (p < .05). Monotherapy can induce significant improvements in pathological and biochemical changes but has not been able to achieve normal patterns. In conclusion, green tea alone has a poor hypoglycaemic effect but can reduce diabetic complications, whereas glibenclamide cannot prevent diabetic complications. The addition of green tea to oral hypoglycaemic therapy has shown a potent synergistic effect.

摘要

我们研究了绿茶对糖尿病肝肾并发症的保护作用。将 30 只雄性 Wistar 大鼠随机分为 5 个相等的组：正常对照组、糖尿病对照组、格列本脲治疗组、绿茶治疗组和联合治疗组；伦理批准号“BERC-014-01-20”。八周后，采用CO ₂安乐死法处死动物，对肝肾组织进行处理并进行病理染色，采集血样进行生化分析。糖尿病大鼠表现出与一些生化参数显着增加相关的多种肝肾形态和细胞凋亡变化，而血清白蛋白和 HDL 与正常对照组相比显着下降（p < .05)。单一疗法可以显着改善病理和生化变化，但无法达到正常模式。总之，单独使用绿茶降糖效果差，但可以减少糖尿病并发症，而格列本脲不能预防糖尿病并发症。在口服降糖疗法中加入绿茶已显示出强大的协同作用。