Abstract

Aim

A high-fat diet (HFD) can lead to obesity and related metabolic disorders. This study evaluated the preventive efficacy of myricetin derivative-rich fraction (MD) from Syzygium malaccense leaf extract against HFD-induced obesity, hyperglycaemia, and oxidative stress in C57BL/6J mice.

Methods

HFD-fed mice were administered MD (50 mg/kg, 100 mg/kg, and 150 mg/kg) or 2 mg/kg metformin (positive control) orally for 16 weeks. Normal diet and HFD-fed control groups received normal saline.

Results

MD dose of 50 mg/kg was better than 100 mg/kg and 150 mg/kg in significantly reducing weight-gain, glucose intolerance, insulin resistance, lipid accumulation in liver and kidney, and improving the serum lipid profile. Lowered protein carbonyls and lipid hydroperoxides in urine and tissue homogenates and elevated reduced glutathione, ferric reducing antioxidant power (FRAP), and Trolox equivalent antioxidant capacity (TEAC) levels in tissue homogenates indicated amelioration of oxidative stress.

Conclusion

MD has therapeutic value in the prevention and management of obesity, hyperglycaemia, and oxidative stress.

中文翻译：

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蒲桃中富含杨梅素衍生物的部分可预防 C57BL/6J 小鼠高脂饮食诱导的肥胖、葡萄糖耐受不良和氧化应激

摘要

目的

高脂肪饮食 (HFD) 会导致肥胖和相关的代谢紊乱。本研究评估了蒲桃叶提取物中富含杨梅素衍生物的部分 (MD)对 C57BL/6J 小鼠 HFD 诱导的肥胖、高血糖和氧化应激的预防功效。

方法

喂食 HFD 的小鼠口服 MD（50 mg/kg、100 mg/kg 和 150 mg/kg）或 2 mg/kg 二甲双胍（阳性对照），持续 16 周。正常饮食和 HFD 喂养的对照组接受生理盐水。

结果

MD剂量50 mg/kg优于100 mg/kg和150 mg/kg，可显着降低体重增加、葡萄糖耐受不良、胰岛素抵抗、肝肾脂质蓄积和改善血脂水平。尿液和组织匀浆中降低的蛋白质羰基和脂质氢过氧化物以及组织匀浆中还原型谷胱甘肽、还原铁抗氧化能力 (FRAP) 和 Trolox 等效抗氧化能力 (TEAC) 水平的升高表明氧化应激得到改善。

结论

MD 在预防和控制肥胖、高血糖和氧化应激方面具有治疗价值。