ABSTRACT Coxiella burnetii and Borrelia burgdorferi sensu lato are intracellular bacteria of great zoonotic importance. To detect these two pathogensin ticks as well as in blood samples, a nested PCR and conventional PCR was used. A total of 525 ticks were collected randomly from infected goats. Blood samples were also collected from tick-infested goats. Both samples were assessed for the presence of these organisms using Polymerase chain reaction (PCR) assays targeting a fragment of 16S rRNA, superoxide dismutase (SOD) of C. burnetii and ospA gene of B. burgdorferi s.l. Sequence analysis of the amplified fragment was used to confirm PCR findings. The PCR products were purified, cloned and custom sequenced. A few blood samples were found positive for both C. burnetii and B. Burgdorferi sensu lato. A total of 12 isolates of the 16S rRNA gene fragment and one isolate of the SOD gene fragment of C. burnetii were obtained. The sequences were aligned and a phylogenetic tree was made with other published C. burnetii sequences. Our finding indicated that ticks were infesting goats as well as blood samples harbour C. burnetii and B. burgdorferi sensu lato providing possible zoonotic transmission of these bacteria from animals to humans through ticks.

中文翻译：

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在印度东北部各州感染山羊的蜱中对伯内氏杆菌和伯氏疏螺旋体的分子检测

摘要 Coxiella burnetii 和 Borrelia burgdorferi sensu lato 是对人畜共患病具有重要意义的细胞内细菌。为了检测蜱以及血液样本中的这两种病原体，使用了巢式 PCR 和常规 PCR。从受感染的山羊中随机收集了总共 525 只蜱。血液样本也从蜱虫感染的山羊身上采集。使用针对 16S rRNA 片段、C.burnetii 的超氧化物歧化酶 (SOD) 和伯氏疏螺旋体 sl 的 ospA 基因的聚合酶链反应 (PCR) 测定评估这两个样品的这些生物体的存在。使用扩增片段的序列分析以确认 PCR 结果。PCR 产物经过纯化、克隆和定制测序。一些血液样本被发现对 C.burnetii 和 B. Burgdorferi sensu lato 均呈阳性。共获得12株16S rRNA基因片段和1株SOD基因片段。对序列进行比对，并使用其他已发表的 C.burnetii 序列制作系统发育树。我们的发现表明，蜱虫感染山羊，血液样本中含有 C.burnetii 和 B. burgdorferi sensu lato，可能通过蜱虫将这些细菌从动物传染给人类。