Adsorption of molecules to the cell walls of microorganisms plays an important role in helping to prevent animal exposure to the toxic and carcinogenic effects of aflatoxins (AFs). The aim of this study was to evaluate the ability of LAB strains, isolated from brewers’ grains, to adsorb aflatoxin B₁ (AFB₁). All LAB were able to reduce the bioavailability of AFB₁ from phosphate buffered-saline (PBS). In addition, the strains retained their effectiveness even after heat treatment. The AFB₁-LAB complex stability was first evaluated through sequential washing steps. These assays demonstrated that a low percentage of AFB₁ was released after consecutive washes. After subjecting the complex to different pH and bile salt treatments, the percentage of bound AF decreased, as compared to the control, but remained at high levels. Finally, to simulate the formation of the AFB₁-LAB complex at conditions similar to those of the gastrointestinal tract, LAB and AFB₁ were homogenized in PBS adjusted at acidic conditions or under different bile salt concentrations. In general, LAB strains showed the highest AFB₁ adsorption at the lowest pH (2) and bile salt concentration (0.05%). In conclusion, the studied strains represent promising biocontrol agents for preventing and/or ameliorating the AFB₁ contamination of feed.

分子对微生物细胞壁的吸附在帮助防止动物暴露于黄曲霉毒素（AFs）的毒性和致癌作用中起着重要作用。这项研究的目的是评估从啤酒糟中分离出的LAB菌株吸附黄曲霉毒素B ₁（AFB ₁）的能力。所有LAB都能降低磷酸盐缓冲盐水（PBS）中AFB ₁的生物利用度。另外，即使在热处理后，菌株仍保持其有效性。首先通过顺序洗涤步骤评估AFB ₁ -LAB复合物的稳定性。这些测定表明，AFB _1的百分比较低在连续洗涤后被释放。在使复合物经历不同的pH和胆盐处理后，与对照相比，结合的AF的百分比降低，但仍保持较高水平。最后，为了模拟在类似于胃肠道的条件下AFB ₁ -LAB复合物的形成，将LAB和AFB ₁在酸性条件下或在不同胆汁盐浓度下调节的PBS中匀浆。通常，LAB菌株在最低pH（2）和胆汁盐浓度（0.05％）时显示出最高的AFB ₁吸附。总之，所研究的菌株代表了用于预防和/或改善饲料中AFB ₁污染的有前途的生物防治剂。