Long noncoding RNA SOX21-AS1 regulates the progression of triple-negative breast cancer through regulation of miR-520a-5p/ORMDL3 axis.,Journal of Cellular Biochemistry

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Long noncoding RNA SOX21-AS1 regulates the progression of triple-negative breast cancer through regulation of miR-520a-5p/ORMDL3 axis.
Journal of Cellular Biochemistry ( IF 3.0 ) Pub Date : 2020-04-10 , DOI: 10.1002/jcb.29674
Xia Liu ₁ , Jingyong Song ₁ , Yu Kang ₁ , Yaojia Wang ₁ , Anyue Chen ₁

Affiliation

Recently, long noncoding RNAs (lncRNAs) have been reported as a new kind of controllers about cancer processes in biology. In spite of the dysregulation of lncRNAs in various kinds of cancers, only a little of the information was effective on the expression configuration and inner effects of lncRNAs in triple‐negative breast cancer (TNBC). This study valued the expression of lncRNA SOX21‐AS1 and the biological role it played in TNBC. In our research, SOX21‐AS1 had a high expression in TNBC cell lines. The functional experiments showed that knockdown of SOX21‐AS1 obviously restrained cell proliferation, migration, invasion, and epithelial‐mesenchymal transition process and promoted cell apoptosis. Mechanistically, SOX21‐AS1 was found to bind with miR‐520a‐5p. Besides, ORMDL3 was identified as a downstream target of miR‐520a‐5p, and the suppressed ORMDL3 expression induced by silenced SOX21‐AS1 could be restored by miR‐520a‐5p inhibition. Further, data from rescue assays revealed that SOX21‐AS1 could regulate the malignancy of TNBC via miR‐520a‐5p/ORMDL3 axis. All in all, we identified that SOX21‐AS1 regulated the cellular process of TNBC cells via antagonizing miR‐520a‐5p availability to upregulate ORMDL3 expression.

中文翻译：

长链非编码 RNA SOX21-AS1 通过调控 miR-520a-5p/ORMDL3 轴调控三阴性乳腺癌的进展。

最近，长链非编码 RNA (lncRNA) 已被报道为生物学中有关癌症过程的新型控制器。尽管 lncRNA 在各种癌症中存在失调，但只有很少的信息对 lncRNA 在三阴性乳腺癌 (TNBC) 中的表达构型和内部效应有效。本研究重视 lncRNA SOX21-AS1 的表达及其在 TNBC 中的生物学作用。在我们的研究中，SOX21-AS1 在 TNBC 细胞系中高表达。功能实验表明，敲低SOX21-AS1可明显抑制细胞增殖、迁移、侵袭和上皮间质转化过程，促进细胞凋亡。从机制上讲，发现 SOX21-AS1 与 miR-520a-5p 结合。此外，ORMDL3 被确定为 miR-520a-5p 的下游靶标，抑制 miR-520a-5p 可以恢复由沉默的 SOX21-AS1 诱导的抑制 ORMDL3 表达。此外，来自救援试验的数据表明，SOX21-AS1 可以通过 miR-520a-5p/ORMDL3 轴调节 TNBC 的恶性程度。总而言之，我们发现 SOX21-AS1 通过拮抗 miR-520a-5p 的可用性来调节 TNBC 细胞的细胞过程，从而上调 ORMDL3 的表达。

更新日期：2020-04-10

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