IRX5 prompts genomic instability in colorectal cancer cells.,Journal of Cellular Biochemistry

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IRX5 prompts genomic instability in colorectal cancer cells.
Journal of Cellular Biochemistry ( IF 3.0 ) Pub Date : 2020-03-11 , DOI: 10.1002/jcb.29693
Xun Sun ₁ , Xinying Jiang ₂ , Jianzhong Wu ₁ , Rong Ma ₁ , Yiqi Wu ₂ , Haixia Cao ₁ , Zhuo Wang ₁ , Siwen Liu ₁ , Junying Zhang ₁ , Yang Wu ₁ , Yuan Zhang ₁ , Jifeng Feng ₁ , Ting Wang ₂

Affiliation

The Iroquois homeobox gene 5 (IRX5), one of the members of the Iroquois homeobox family, has been identified to correlate with worse prognosis in many cancers, including colorectal cancer (CRC). In this study, upregulation of IRX5 revealed a great reduction in the proliferation of CRC colorectal cancer cell line SW480 and DLD‐1, which was accompanied by G1/S arrest, increased expression in cyclin E1, P21, and P53 and a decrease in cyclin A2, B1, and D1. Furthermore, IRX5‐mediated an increase expression of RH2A protein, the biomarker of DNA damage. Consequently, the SA‐β‐gal level is higher in IRX5‐overexpression cells compared to control ones, which showed elevated DNA damage triggered cellular senescence. Recapitulating the above findings, IRX5 exhibited higher levels of genomic instability. IRX5 may be a perspective target for cancer therapy and it deserves further investigation.

中文翻译：

IRX5 提示结直肠癌细胞的基因组不稳定性。

易洛魁族同源框基因 5 (IRX5) 是易洛魁族同源框家族的成员之一，已被确定与包括结直肠癌 (CRC) 在内的许多癌症的不良预后相关。在这项研究中，IRX5 的上调揭示了 CRC 结直肠癌细胞系 SW480 和 DLD-1 的增殖大大减少，伴随着 G1/S 期停滞，细胞周期蛋白 E1、P21 和 P53 的表达增加以及细胞周期蛋白的减少。 A2、B1 和 D1。此外，IRX5 介导了 RH2A 蛋白（DNA 损伤的生物标志物）的表达增加。因此，与对照细胞相比，IRX5 过表达细胞中的 SA-β-gal 水平更高，这表明 DNA 损伤升高引发了细胞衰老。概括上述发现，IRX5 表现出更高水平的基因组不稳定性。

更新日期：2020-03-11

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