The TCP4 transcription factor plays an important role in plant growth and development, especially in flower development. PmTCP4 is involved in the process of pistil abortion in Japanese apricot, but its molecular mechanism, particularly the DNA binding sites and co‐regulatory genes, are quite unknown. Therefore, to identify the genome‐wide binding sites of PmTCP4 transcription factors and their co‐regulatory genes, chromatin immunoprecipitation sequencing (ChIP‐Seq) was carried out. ChIP‐Seq data produced the maximum enriched peaks in two Japanese apricot cultivars ‘Daqiandi’ (DQD) and ‘Longyan’ (LY), which showed that the majority of DNA‐protein interactions are relevant and have a significant function in binding sites. Moreover, 720 and 251 peak‐associated genes regulated by PmTCP4 were identified in DQD and LY, respectively, and most of them were involved in the flower and pistil development process. Furthermore, Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis showed that photosynthesis and oxidative phosphorylation were the most enriched pathways in both cultivars and all identified genes related to these pathways were down‐regulated. This study will provide a reference for a better understanding of the PmTCP4 regulatory mechanism during pistil abortion in Japanese apricot.

中文翻译：

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ChIP-Seq在日本杏子雌性流产期间对PmTCP4转录因子结合位点进行全基因组分析

TCP4转录因子在植物生长和发育，特别是在花卉发育中起重要作用。Pm TCP4参与了日本杏的雌蕊流产过程，但其分子机制，尤其是DNA结合位点和共调控基因，尚不清楚。因此，为了鉴定Pm TCP4转录因子及其共调控基因的全基因组结合位点，我们进行了染色质免疫沉淀测序（ChIP-Seq）。ChIP-Seq数据在两个日本杏品种``大千di''（DQD）和``龙岩''（LY）中产生了最大富集峰，这表明大多数DNA-蛋白质相互作用是相关的，并且在结合位点上具有重要作用。此外，受峰值调控的720和251个与峰值相关的基因在DQD和LY中分别鉴定到Pm TCP4，并且大多数与花和雌蕊发育过程有关。此外，《京都基因与基因组百科全书》（KEGG）分析表明，光合作用和氧化磷酸化是两个品种中最丰富的途径，所有与这些途径相关的已鉴定基因均被下调。该研究将为更好地了解日本杏雌蕊人工流产过程中Pm TCP4调控机制提供参考。