Soil microbiome is one of the most heterogeneous biological systems. State‐of‐the‐art molecular approaches such as those based on single‐amplified genomes (SAGs) and metagenome assembled‐genomes (MAGs) are now improving our capacity for disentailing soil microbial‐mediated processes. Here, we analysed publicly available datasets of soil microbial genomes and MAG's reconstructed from the Amazon's tropical soil (primary forest and pasture) and active layer of permafrost, aiming to evaluate their genome size. Our results suggest that the Candidate Phyla Radiation (CPR)/Patescibacteria phyla have genomes with an average size fourfold smaller than the mean identified in the RefSoil database, which lacks any representative of this phylum. Also, by analysing the potential metabolism of 888 soil microbial genomes, we show that CPR/Patescibacteria representatives share similar functional profiles, but different from other microbial phyla and are frequently neglected in the soil microbial surveys. Finally, we argue that the use of MAGs may be a better choice over SAGs to expand the soil microbial databases, like RefSoil.

中文翻译：

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元基因组组装基因组揭示了土壤中CPR /门扇杆菌的相似功能。

土壤微生物组是最异质的生物系统之一。先进的分子方法，例如基于单扩增基因组（SAG）和元基因组组装基因组（MAG）的方法，现在正在提高我们消除土壤微生物介导过程的能力。在这里，我们分析了土壤微生物基因组和从亚马逊热带土壤（原始森林和牧场）以及多年冻土的活动层重建的MAG的公开可用数据集，旨在评估它们的基因组大小。我们的研究结果表明，候选门生辐射（CPR）/细菌门菌的基因组平均大小比RefSoil数据库中鉴定的平均值小四倍，而该数据库没有该门菌的任何代表。此外，通过分析888个土壤微生物基因组的潜在代谢，我们显示，CPR / Patecibacteria代表共享相似的功能配置文件，但与其他微生物门不同，并且在土壤微生物调查中经常被忽略。最后，我们认为，使用MAGs可能是优于SAG扩展土壤微生物数据库（如RefSoil）的更好选择。