Clinostomus (Leuciscidae) is a wide‐ranging freshwater fish genus that occurs throughout eastern North America and southern portions of Canada with two species currently recognized: C. elongatus and C. funduloides. A previous taxonomic study of C. funduloides recognized two subspecies (estor and funduloides) and one diagnosed, but undescribed subspecies based on morphological characteristics and geographic distribution. In this study, we used three molecular markers (cytochrome b, S7 intron 1 and growth hormone intron 4) to test the three lineage hypothesis and evaluate genetic variation of C. funduloides across the range using Bayesian inference. Our results indicate that C. funduloides is not monophyletic, as individuals of C. elongatus nest within C. funduloides in both the mtDNA and nDNA phylogenetic analyses, although the position of C. elongatus varies between data sets. In addition, some of the recovered clades are deeply divergent from one another, further supporting the distinctiveness of many of the populations. Overall, these results suggest that subspecies designations are not warranted and a taxonomic revision is needed as Clinostomus is likely more diverse than is currently recognized.

中文翻译：

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评估罗斯赛德D（Teleostei，Leuciscidae）（一种形态广泛可变的分类群）的系统地理学变异

斜纹鱼类（Linociscidae）是一种广泛的淡水鱼类属，分布于北美东部和加拿大南部，目前有两种已知的物种：加长线虫（C. elongatus）和C.funduloides。先前对C.funduloides的分类学研究确认了两个亚种（estor和funduloides），并且一个已诊断，但根据形态特征和地理分布未描述。在这项研究中，我们使用了三种分子标记（细胞色素b，S7内含子1和生长激素内含子4）来检验这三个谱系假说并评估角膜梭菌的遗传变异。使用贝叶斯推断在整个范围内。我们的研究结果表明，C. funduloides不是单系，作为个人C.细长内巢C. funduloides同时在mtDNA与nDNA的系统发育分析，虽然位置C.细长数据组之间变化。此外，一些已恢复的进化枝彼此之间有很大的分歧，进一步支持了许多种群的独特性。总体而言，这些结果表明，亚种的命名没有必要，并且需要对分类进行修订，因为斜纹梭菌可能比目前公认的更加多样化。