Enhancing organophosphate hydrolase efficacy via protein engineering and immobilization strategies,Annals of the New York Academy of Sciences

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Enhancing organophosphate hydrolase efficacy via protein engineering and immobilization strategies
Annals of the New York Academy of Sciences ( IF 4.1 ) Pub Date : 2020-08-19 , DOI: 10.1111/nyas.14451
Priya Katyal ₁ , Stanley Chu ₁ , Jin Kim Montclare _{1,

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Affiliation

Organophosphorus compounds (OPs), developed as pesticides and chemical warfare agents, are extremely toxic chemicals that pose a public health risk. Of the different detoxification strategies, organophosphate‐hydrolyzing enzymes have attracted much attention, providing a potential route for detoxifying those exposed to OPs. Phosphotriesterase (PTE), also known as organophosphate hydrolase (OPH), is one such enzyme that has been extensively studied as a catalytic bioscavenger. In this review, we will discuss the protein engineering of PTE aimed toward improving the activity and stability of the enzyme. In order to make enzyme utilization in OP detoxification more favorable, enzyme immobilization provides an effective means to increase enzyme activity and stability. Here, we present several such strategies that enhance the storage and operational stability of PTE/OPH.

中文翻译：

通过蛋白质工程和固定化策略增强有机磷酸酯水解酶的功效

作为杀虫剂和化学战剂而开发的有机磷化合物 (OP) 是一种毒性极强的化学品，会对公共健康构成威胁。在不同的解毒策略中，有机磷水解酶引起了广泛关注，为暴露于 OPs 的人解毒提供了潜在途径。磷酸三酯酶 (PTE)，也称为有机磷酸酯水解酶 (OPH)，是一种作为催化生物清除剂被广泛研究的酶。在这篇综述中，我们将讨论旨在提高酶活性和稳定性的 PTE 蛋白质工程。为了使酶在OP解毒中的利用更加有利，酶固定化提供了一种提高酶活性和稳定性的有效手段。这里，

更新日期：2020-08-19

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