当前位置: X-MOL 学术J. Sci. Adv. Mater. Dev. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Bioreduction synthesis of zinc oxide nanoparticles using Delonix regia leaf extract (Gul Mohar) and its agromedicinal applications
Journal of Science: Advanced Materials and Devices ( IF 6.7 ) Pub Date : 2020-12-01 , DOI: 10.1016/j.jsamd.2020.07.009
J.P. Shabaaz Begum , K. Manjunath , S. Pratibha , N. Dhananjaya , Prashant Sahu , Sushil Kashaw

Abstract The bioreduction of Zinc nitrate hexahydrate using crude leaf extract of Gulmohar, by combustion method at 400 °C is carried out to form the ZnO nanostructures. They were characterized by UV- Visible spectroscopy, XRD analysis, FTIR spectroscopy, SEM, and TEM analysis for the structural and morphological details. The antibacterial activity of pure wurtzite ZnO NPs was carried out by the Resazurin plate assay method followed by antifungal evaluation utilizing the food poisoning technique using bavistin as a positive control. The anticancer potential was performed using the HeLa cell line for the quantitative evaluation of MTT and apoptosis assay. Antibacterial effect of ZnO NPs on Escherichia coli and Staphylococcus aureus was in the range 0.25–0.0025 μg/ml and antifungal effect on Fusarium oxysporum and Phomposis azadirachtae was in the range of 100–700 μg/ml. The MTT assay and apoptosis analysis on the HeLa cell line particularized optimal cell uptake and cell toxicity on 24 h of exposure. This biogenic method based ZnO NPs synthesis flags the way indicating the significant microbicidal potential against selected plant and animal pathogenic microorganisms and also proved to have an optimal cytotoxic effect against HeLa cell lines. Hence acts as promising nanoantibiotics for application in medicine.

中文翻译:

使用 Delonix regia 叶提取物 (Gul Mohar) 生物还原合成氧化锌纳米颗粒及其农业医学应用

摘要 使用古尔莫哈尔的粗叶提取物,通过燃烧法在 400 °C 下生物还原六水合硝酸锌以形成 ZnO 纳米结构。它们通过紫外-可见光谱、XRD 分析、FTIR 光谱、SEM 和 TEM 分析来表征结构和形态细节。纯纤锌矿 ZnO NPs 的抗菌活性通过刃天青板测定法进行,然后使用食物中毒技术进行抗真菌评估,使用巴维汀作为阳性对照。抗癌潜力是使用 HeLa 细胞系进行的,用于 MTT 和细胞凋亡测定的定量评估。ZnO NPs 对大肠杆菌和金黄色葡萄球菌的抗菌作用在 0.25-0 范围内。0025 μg/ml 和对尖孢镰刀菌和印楝的 Phomposis azadirachtae 的抗真菌作用在 100-700 μg/ml 的范围内。对 HeLa 细胞系的 MTT 测定和细胞凋亡分析明确了暴露 24 小时时的最佳细胞摄取和细胞毒性。这种基于 ZnO NPs 合成的生物方法标志着对选定的植物和动物病原微生物具有显着杀微生物潜力的方式,并且还证明对 HeLa 细胞系具有最佳的细胞毒作用。因此,作为有前途的纳米抗生素在医学上的应用。这种基于 ZnO NPs 合成的生物方法标志着对选定的植物和动物病原微生物具有显着杀微生物潜力的方式,并且还证明对 HeLa 细胞系具有最佳的细胞毒作用。因此,作为有前途的纳米抗生素在医学上的应用。这种基于 ZnO NPs 合成的生物方法标志着对选定的植物和动物病原微生物具有显着杀微生物潜力的方式,并且还证明对 HeLa 细胞系具有最佳的细胞毒作用。因此,作为有前途的纳米抗生素在医学上的应用。
更新日期:2020-12-01
down
wechat
bug