Plant parasitic nematodes (PPNs) cause huge agricultural and silvicultural losses every year. Most studies that investigated various mechanisms for killing nematodes used the model organism Caenorhabditis elegans. Pseudomonas chlororaphis is an important biocontrol bacterium with great application potential in agriculture. Pseudomonas chlororaphis strains have the potential to control PPNs. In our previous study, we obtained one P. chlororaphis-like bacterium, namely, SPS-41, from the rhizosphere of sweet potato. This strain exhibited wide-spectrum of antifungal activity. In the present study, the nematicidal activity of the P. chlororaphis SPS-41 strain against C. elegans was examined. This strain was identified as Pseudomonas chlororaphis subsp. aureofaciens via 16S rRNA phylogenetic analysis. Results showed that this strain could kill nematodes in a short time on both fast- and slow-media. Both the liquid culture and volatile organic compounds (VOCs) produced by this strain exhibited strong nematicidal activity. VOCs fumigation by the SPS-41 strain triggered the immune response of C. elegans. A total of 23 VOCs from this strain were analyzed via solid-phase microextraction gas chromatography–mass spectrometry. Seven major VOCs were picked to test its nematicidal activity. Four of these VOCs, namely, 2-methyl-1-butanol, octanoic acid ethyl ester, ethyl acetate, and isobutyl acetate, could kill nematodes. Octanoic acid ethyl ester displayed the strongest nematocidal activity. The results suggested that the SPS-41 strain and its VOCs can be used as a new strategy for controlling nematodes.

植物寄生线虫（PPN）每年造成巨大的农业和造林损失。大多数研究杀死线虫的各种机制的研究都使用模型生物秀丽隐杆线虫。绿假单胞菌是一种重要的生物防治细菌，在农业上具有巨大的应用潜力。绿假单胞菌菌株具有控制PPN的潜力。在我们以前的研究中，我们从甘薯的根际获得了一种类似于绿腐霉的细菌，即SPS-41。该菌株表现出广谱的抗真菌活性。在本研究中，P。chlororaphis SPS-41菌株对秀丽隐杆线虫的杀线虫活性被检查了。该菌株被鉴定为绿假单胞菌亚种。通过16S rRNA系统发育分析分析金黄色葡萄球菌。结果表明，该菌株可以在短时间内在快速和慢速培养基上杀死线虫。该菌株产生的液体培养物和挥发性有机化合物（VOC）均显示出强大的杀线虫活性。SPS-41菌株对VOC的熏蒸触发线虫的免疫反应。通过固相微萃取气相色谱-质谱法分析了该菌株中的23种VOC。选择了七个主要的挥发性有机化合物来测试其杀线虫活性。这些挥发性有机化合物中的四种，即2-甲基-1-丁醇，辛酸乙酯，乙酸乙酯和乙酸异丁酯，可以杀死线虫。辛酸乙酯显示出最强的杀线虫活性。结果表明，SPS-41菌株及其VOC可用作控制线虫的新策略。