Cell transplantation into immunodeficient recipients is a widely used approach to study stem cell and cancer biology; however, studying cell states post transplantation in vivo is inconvenient in mammals. Here, we generated a foxn1/Casper mutant zebrafish that is transparent and exhibits T cell deficiency. By employing the line for hematopoietic stem cell (HSC) transplantation (HSCT), we could achieve nonconditioned transplantation. Meanwhile, we found that fetal HSCs from 3 days post fertilization zebrafish embryos produce a better transplant outcome in foxn1/Casper mutants, compared with adult HSCs. In addition to HSCT, the foxn1/Casper mutant is feasible for allografts of myelodysplastic syndrome-like and muscle cells, as well as xenografts of medaka muscle cells. In summary, foxn1/Casper mutants permit the nonconditioned engraftment of multiple cell types and visualized characterization of transplanted cells in vivo.

将细胞移植到免疫缺陷受体中是研究干细胞和癌症生物学的一种广泛使用的方法。然而，在哺乳动物体内研究移植后的细胞状态并不方便。在这里，我们生成了一个foxn1 / Casper突变斑马鱼，它是透明的并表现出T细胞缺陷。通过将该线用于造血干细胞（HSC）移植（HSCT），我们可以实现非条件移植。同时，我们发现，与成年HSC相比，受精斑马鱼胚胎后3天的胎儿HSC在foxn1 / Casper突变体中产生了更好的移植结果。除了HSCT，foxn1 / Casper该突变体对于骨髓增生异常综合征样和肌细胞的异体移植以及高肌细胞的异种移植是可行的。总而言之，foxn1 / Casper突变体允许多种细胞类型的非条件植入和体内移植细胞的可视化表征。