当前位置: X-MOL 学术Sens. Actuators A Phys. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Wavelength-dependent angular shift and figure of merit of silver-based surface plasmon resonance biosensor
Sensors and Actuators A: Physical ( IF 4.1 ) Pub Date : 2020-08-20 , DOI: 10.1016/j.sna.2020.112289
Sugandh Priya , Ranjit Laha , Venkata Ramanaiah Dantham

Herein, we report the dependence of the angular shift (Δθ) and figure of merit (FOM) of the surface plasmon resonance (SPR) biosensor on the excitation wavelength of light (λ) studied using a developed multi-wavelength two prism optical configuration. The experiments have been performed with Bovine Serum Albumin (BSA) and Cytochrome C protein molecules. The λ range was varied from visible to near-infrared. The role of concentration of the biomolecules on the SPR sensitivity has been studied systematically. The experimental results have been compared with theoretical results based on the transfer matrix method. From these results, the value of Δθ is found significantly larger for lower λ values and it is nearly independent of the thickness of the film. However, for a fixed concentration of biomolecules, the FOM of the SPR biosensor is found nearly insensitive to the λ values. Moreover, the role of λ on the characteristic parameters such as depth and FWHM of the resonance has been studied systematically.



中文翻译:

银基表面等离子体共振生物传感器的波长相关角位移和品质因数

在这里,我们报告了表面等离子体激元共振(SPR)生物传感器的角位移(Δθ)和品质因数(FOM)对使用已开发的多波长两棱镜光学配置研究的光的激发波长(λ)的依赖性。实验是用牛血清白蛋白(BSA)和细胞色素C蛋白分子进行的。λ范围从可见光到近红外不等。已经系统地研究了生物分子的浓度对SPR敏感性的作用。将实验结果与基于传递矩阵法的理论结果进行了比较。根据这些结果,发现对于较低的λ值,Δθ的值明显更大,并且几乎与膜的厚度无关。但是,对于固定浓度的生物分子,发现SPR生物传感器的FOM对λ值几乎不敏感。此外,系统地研究了λ对共振深度和FWHM等特征参数的作用。

更新日期:2020-08-20
down
wechat
bug