Extracellular DNA Trap (ET) formation by granulocyte is a strong innate immune machinery that plays crucial roles in trapping and killing of pathogens. Here, we show Eosinophil Extracellular DNA Trap (EET) formation in goats naturally infected with nodular worms (Oesophagostomum columbianum, Strongyloidae: Nematoda). By a slaughterhouse based survey, we found that 60% goats were infected with nodular worms. We detected numerous, hard and pale yellow to dark black nodules of variable sizes (0.25–2 cm) in the large intestine and the number of nodules were significantly (p < .05) higher in the cecum (21.7 ± 17.9) than in the colon (10.1 ± 9.9). Histologically, pink colored circumscribed caseous mass was surrounded by a dense zone of infiltration and fibrous proliferation along with massive infiltration of eosinophils in and around the necrotic mass. DAPI staining revealed huge accumulation of extracellular DNA, which formed wide ridge like structure surrounding the necrotic zone. Massive release of eosinophils cationic proteins (ECP), a helmintho-toxic substance, was found into the lesions. Collectively, our results suggest that nodular worm infection induces EETosis and ECP release, and is one of the major parasitic problem affecting Black Bengal goats that causes distortion of normal architecture of the gut wall.

粒细胞形成的细胞外DNA陷阱（ET）是一种强大的先天免疫机制，在捕获和杀死病原体中起着至关重要的作用。在这里，我们显示了自然感染结节性蠕虫（Oesophagostomum columbianum的山羊）中嗜酸性粒细胞外DNA陷阱（EET）的形成，Strongyloidae：线虫）。通过基于屠宰场的调查，我们发现60％的山羊感染了结节性蠕虫。我们在大肠中检测到许多大小不一的硬，浅黄色至深黑色至大小不等的结节（0.25–2 cm），盲肠（21.7±17.9）中结节的数量明显高于（21.7±17.9）（p <0.05）。结肠（10.1±9.9）。从组织学上看，粉红色的外接干酪样肿块周围有致密的浸润和纤维增生区，以及坏死肿块内和周围的嗜酸性粒细胞大量浸润。DAPI染色显示大量的细胞外DNA积累，形成围绕坏死区的宽脊状结构。在病变中发现了嗜酸性粒细胞阳离子蛋白（ECP）的大量释放，这是一种对蠕虫有毒的物质。总的来说，