This study first time depicts a synthetic seed protocol of Hedychium coronarium J. Koenig by alginate encapsulation. A non-embryogenic type of propagule i.e. axenic shoot segments excised from in vitro proliferated shoot cultures were used for preparation of synthetic seeds. The production and conversion (one-step plantlet formation) of synthetic seeds was influenced by the concentrations of sodium alginate and calcium chloride (CaCl₂) used. The combination of 3% sodium alginate and 100 mM CaCl₂ was optimum encapsulation matrix showing highest conversion rate (90%) of synthetic seeds on Murashige and Skoog’s (MS) basal medium. The synthetic seeds were stored up to 4 weeks at two different temperatures, one at low temperature (4 °C) and another at culture room condition (25 °C) with one-step conversion rate of 73% and 62% respectively. The clonal fidelity of the plants produced from both freshly prepared synthetic seeds and those synthetic seeds stored for 4 weeks at 4 °C and 25 °C were confirmed using two molecular markers namely randomly amplified polymorphic DNA (RAPD) and inter simple sequence repeats (ISSR). This synthetic seed production protocol of H. coronarium could be utilized for its propagation as well as transfer and exchange of germplasm to distance places.

这项研究首次描述了通过藻酸盐包封法合成冠花Hedychium coronarium J. Koenig的合成种子方案。非胚发生型的繁殖体，即从体外增殖的芽培养物中切下的轴生芽段被用于制备合成种子。海藻酸钠和氯化钙（CaCl ₂）的浓度影响合成种子的生产和转化（一步步成苗）。3％海藻酸钠和100 mM CaCl _2的组合最佳包封基质在Murashige和Skoog（MS）基础培养基上显示出最高的合成种子转化率（90％）。合成种子在两种不同的温度下最多可保存4周，一种在低温（4°C）下，另一种在培养室条件（25°C）下，一步转化率分别为73％和62％。使用两个分子标记即随机扩增的多态性DNA（RAPD）和内部简单序列重复（ISSR）确认了新鲜制备的合成种子以及在4°C和25°C下储存4周的合成种子生产的植物的克隆保真度。 ）。冠状杆菌的这种合成种子生产方案可用于其繁殖以及种质向远处的转移和交换。