The information concerning the effects of single and combined exposure to cocaine (COC) and its main metabolite, the benzoylecgonine (BE), towards marine organisms is still scant. Thus, the aim of this work was to compare the effects induced by 96 -hs exposure to a concentration of COC (500 ng/L) or BE (20 ng/L) and their mixture (500 ng/L of COC and 20 ng/L of BE) on Mytilus galloprovincialis. Oxidative stress biomarkers were applied on mussel gills and digestive gland, investigating changes in the amount of reactive oxygen species, activity of antioxidant (SOD, CAT and GPx) and detoxifying (GST) enzymes and lipid peroxidation. Independent exposure to COC and BE slightly altered mussel oxidative status in both the organs, while the mixture induced more marked responses compared to single molecules. Our results suggest the necessity to explore the toxicity of illicit drug mixtures to shed light on the risk of these molecules to marine organisms.

关于可卡因（COC）及其主要代谢产物苯甲酰芽子碱（BE）一次或多次接触对海洋生物的影响的信息仍然很少。因此，这项工作的目的是比较96小时暴露于浓度为COC（500 ng / L）或BE（20 ng / L）及其混合物（500 ng / L COC和20 ng / L）在Mytilus galloprovincialis上。将氧化应激生物标记物应用于贻贝和消化腺，研究活性氧的数量，抗氧化剂（SOD，CAT和GPx）的活性以及解毒（GST）酶和脂质过氧化的变化。独立暴露于COC和BE会轻微改变两个器官中的贻贝氧化状态，而与单分子相比，该混合物会引起更多明显的反应。我们的结果表明，有必要探索非法药物混合物的毒性，以阐明这些分子对海洋生物的风险。