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Comparative evaluation of assays for IgM detection of rubella and measles infections
Enfermedades Infecciosas y Microbiología Clínica ( IF 2.6 ) Pub Date : 2020-08-20 , DOI: 10.1016/j.eimc.2020.06.019
Mayte Pérez Olmeda 1 , Pilar Balfagón 1 , Juan Camacho 1 , Desirée Dafouz 1 , Jesús de la Fuente 1 , María Ángeles Murillo 1 , José Luis Muñoz 1 , Aurora Fernández García 2 , Juan Carlos Sanz 3 , Fernando de Ory 4
Affiliation  

Background

Serological diagnosis of infections due to measles and rubella viruses is done by IgM detection. The aim of this study was to comparatively evaluate commercial systems for detecting IgM against both viruses, including those of ELISA, in indirect and capture formats, chemiluminescence and electrochemiluminescence.

Methods

Seven (for rubella) and six (for measles) assays were studied. One hundred and sixty two samples were included in the study (from 90 rubella and 72 measles cases), and all were analyzed in all the assays.

Results

The ranges of sensitivity, specificity and agreement for rubella were 94.8–100%, 52.4–100% and 75.5–98.1%, respectively. The corresponding ranges for measles assays were 87.0–100%, 53.3–100%, and 73.0–99.4%.

Conclusion

The best-performing assays were chemiluminescence (for measles and rubella IgM), and electrochemiluminescence (for rubella IgM).



中文翻译:


风疹和麻疹感染 IgM 检测方法的比较评估


 背景


麻疹和风疹病毒感染的血清学诊断是通过 IgM 检测来完成的。本研究的目的是比较评估用于检测两种病毒 IgM 的商业系统,包括间接和捕获格式、化学发光和电化学发光的 ELISA 系统。

 方法


研究了七种(风疹)和六种(麻疹)检测方法。该研究纳入了 162 个样本(来自 90 个风疹病例和 72 个麻疹病例),并在所有检测中对所有样本进行了分析。

 结果


风疹的敏感性、特异性和一致性范围分别为 94.8-100%、52.4-100% 和 75.5-98.1%。麻疹检测的相应范围为 87.0–100%、53.3–100% 和 73.0–99.4%。

 结论


效果最好的检测是化学发光(针对麻疹和风疹 IgM)和电化学发光(针对风疹 IgM)。

更新日期:2020-08-20
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