The aim of this study was to evaluate the inflammatory (peripheral and lipopolysaccharide (LPS)-stimulated released from whole blood) and metabolic (glucose and insulin) profile of inactive obese men in response to two isoenergetic models of aerobic exercise training (~300 kcal each exercise session). Twenty-two participants (28.7 ± 1.6 years; BMI = 34.4 ± 0.1 kg/m2) were randomized into two groups: I) HIIT: high-intensity interval training (10× 1 bout: 1 min - 100% Maximal Aerobic Velocity) or II) MICT: moderate-intensity continuous training (65% Maximal Aerobic Velocity; kcal equal to HIIT). Both groups trained three times per week for 6-weeks. Fasting blood samples were collected before and 0, 30, and 60 min after exercise during the first and last training sessions for evaluation of: I) MIP-1ɑ, insulin, glucose, visceral and subcutaneous fat depots, oral glucose tolerance test, and homeostatic model assessment of insulin resistance (HOMA-IR) index; II) Peripheral (TNF-α, IL-6, and IL-10) and LPS-stimulated release of TNF-α and IL-10 were analyzed before, 0, and 60 min after sessions. IL-6 concentration remained elevated up to 60-min after the acute exercise session (p < 0.001), and IL-10 concentration was higher after 30 and 60-min (p = 0.001) compared to rest, independent of training period and protocol. AUC of IL-10 presented effect of type of training (p = 0.023) with MICT group showed significantly higher values than the HIIT. The ex-vivo assay showed higher IL-10 secretion in response to LPS immediately (p = 0.003) after both acute MICT and HIIT exercise sessions, independent of training period. Fifteen subjects presented decreased HOMA-IR after 6-weeks and seven presented an increase in this index. When we excluded the two least responsive subjects, it was possible to observe a decrease in HOMA-IR (p = 0.020) after training. Taken together, our results suggest that both HIIT and MICT (with same energy expenditure) promote similar effects on HOMA-IR and led to elevations in IL-10 production in LPS-stimulated whole blood, suggesting that leukocytes had an enhanced ability to secrete anti-inflammatory cytokines after the exercise bout.

中文翻译：

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高强度和中等强度的训练改变了肥胖男性在急性运动后 LPS 诱导的体外白细胞介素 10 的产生

本研究的目的是评估非活动性肥胖男性对两种等能有氧运动训练模型（~300 kcal每次练习）。22 名参与者（28.7 ± 1.6 岁；BMI = 34.4 ± 0.1 kg/m2）被随机分为两组：I) HIIT：高强度间歇训练（10×1 回合：1 分钟 - 100% 最大有氧速度）或II) MICT：中等强度的持续训练（65% 最大有氧速度；kcal 等于 HIIT）。两组每周训练 3 次，为期 6 周。在第一次和最后一次训练期间，在运动前和运动后 0、30 和 60 分钟收集空腹血样，用于评估：I) MIP-1ɑ、胰岛素、葡萄糖、内脏和皮下脂肪库、口服葡萄糖耐量试验和胰岛素抵抗（HOMA-IR）指数的稳态模型评估；II) 外周（TNF-α、IL-6 和 IL-10）和 LPS 刺激的 TNF-α 和 IL-10 的释放在会话前、0 和 60 分钟后进行了分析。IL-6 浓度在急性运动后 60 分钟内保持升高（p < 0.001），与休息相比，30 和 60 分钟后 IL-10 浓度更高（p = 0.001），与训练时间和方案无关. IL-10 的 AUC 呈现出训练类型的影响 (p = 0.023)，MICT 组显示出显着高于 HIIT 的值。体外试验显示，在急性 MICT 和 HIIT 锻炼后，立即对 LPS 产生更高的 IL-10 分泌（p = 0.003），与训练时间无关。6 周后 15 名受试者的 HOMA-IR 降低，7 名受试者的该指数增加。当我们排除两个反应最差的受试者时，可以观察到训练后 HOMA-IR (p = 0.020) 的下降。总之，我们的结果表明 HIIT 和 MICT（具有相同的能量消耗）对 HOMA-IR 的促进作用相似，并导致 LPS 刺激的全血中 IL-10 的产生升高，表明白细胞具有增强的分泌抗- 运动后的炎症细胞因子。