Cryopreservation of clonal plant germplasm is a reliable way to preserve important agronomic traits and protect against loss of crop genetic diversity of many horticultural species. Dormant bud cryopreservation techniques present an efficient alternative to the labor-intensive shoot tip cryopreservation process and may allow a single technician to preserve large quantities of germplasm in a season. This method of cryopreservation takes advantage of the natural dormancy in cold hardy crops, making it a viable technique for only deciduous trees and shrubs. Many factors must be considered when attempting to perform dormant bud research methods to applied-level germplasm preservation efforts. This process is necessarily a seasonal endeavor, which puts strain on labor and facilities particularly in winter. Integration of methods and procedures using different crop species or new equipment provides additional challenges that must be tested in advance. By identifying variables of dormant bud processing in cryopreservation literature, options emerge that allow for the modification of reported methods to work within the confines of institutional resources. Infrastructure, pre-processing, and recovery stages are discussed in terms of necessity and available alternatives to allow informed decision making in establishing an applied dormant budwood genebank.

冷冻保存植物种质是一种可靠的方法，可以保留重要的农艺性状并防止许多园艺物种的作物遗传多样性丧失。休眠芽冷冻保存技术是劳动密集型芽尖冷冻保存方法的一种有效替代方法，并且可以允许单个技术人员在一个季节内保存大量种质。这种低温保存方法利用了耐寒作物的自然休眠特性，使其成为仅对落叶乔木和灌木可行的技术。尝试对应用级种质保存措施进行休眠芽研究方法时，必须考虑许多因素。此过程必定是季节性的工作，特别是在冬天，这会给劳动力和设施造成压力。使用不同作物品种或新设备整合方法和程序会带来其他挑战，必须事先进行测试。通过在冷冻保存文献中确定休眠芽处理的变量，出现了一些选择，这些选项允许修改报告的方法以在机构资源的范围内起作用。基础设施，预处理和恢复阶段将根据必要性和可用替代方法进行讨论，以允许在建立应用的休眠芽木种质库时进行明智的决策。