Detection of (1,3)-beta-d-glucan (BDG), a component of the cell wall of many fungi, was studied in bronchoalveolar lavage fluid (BALF) as a possible aid for the diagnosis of proven/probable invasive pulmonary aspergillosis (IPA). BDG was measured on stored BALF from 13 patients with EORTC/MSGERC defined proven/probable IPA and 26 matched control patients without IPA. The median BALF BDG was 80 pg/mL (range < 45–8240 pg/mL) in the IPA cohort and 148 pg/mL (range < 45–5460 pg/mL) in the non-IPA cohort. Using a positive cutoff of ≥ 80 pg/mL, sensitivity was 54% and specificity was 38%. Higher cutoff values led to improvement in specificity but a dramatic decrease in sensitivity. ROC/AUC analysis was unable to identify an optimal cutoff value at which test performance was enhanced: AUC 0.43, 95% CI 0.24–0.63. When the BDG assay was performed on BALF, neither sensitivity nor specificity was sufficient for use in the diagnosis of IPA.

检测（1,3）-beta- d在支气管肺泡灌洗液（BALF）中研究了β-葡聚糖（BDG），它是许多真菌的细胞壁成分，可作为诊断已确诊/可能的侵袭性肺曲霉病（IPA）的可能手段。在13例EORTC / MSGERC定义的确诊/可能IPA患者和26例无IPA的对照患者中，对储存的BALF进行BDG测量。在IPA队列中，BALF BDG的中位数为80 pg / mL（范围<45–8240 pg / mL），在非IPA队列中，其中位数为148 pg / mL（范围<45-5460 pg / mL）。使用≥80 pg / mL的阳性截止值，灵敏度为54％，特异性为38％。更高的临界值导致特异性提高，但灵敏度急剧下降。ROC / AUC分析无法确定可提高测试性能的最佳临界值：AUC 0.43，95％CI 0.24-0.63。在BALF上进行BDG分析时，