Abstract

Chronic myeloid leukemia is a clonal hematopoietic stem cell disease characterized by myeloid expansion. The hallmark of the disease is the Philadelphia chromosome, which results in the formation of the BCR-ABL oncogene, a tyrosine kinase that is involved in many signaling pathways including Wnt signaling. The latter has a unique role in chronic myeloid leukemia progression; activated signaling leads to the establishment of an additional leukemic stem cell population derived from granulocyte-macrophage progenitors. sFRP1 is an inhibitor of Wnt signaling and its expression is important for differentiation and maintenance of hematopoietic stem cells. In this study, we aimed to investigate miRNAs that target Wnt signaling by being co-induced along with the expression of sFRP1 in K562 cells. We present a detailed analysis of hsa-mir-221-3p, hsa-mir-222-3p, hsa-mir-106b-5p, hsa-let-7f-5p, hsa-mir-182-5p, hsa-mir-191-5p, and hsa-mir-183-5p and their target genes and their involvement in Wnt signaling.

中文翻译：

---

sFRP1表达诱导miRNA调节慢性粒细胞白血病细胞中的Wnt信号。

摘要

慢性粒细胞性白血病是一种以粒细胞扩张为特征的克隆性造血干细胞疾病。该疾病的标志是费城染色体，其导致BCR-ABL的形成癌基因，一种酪氨酸激酶，参与许多信号通路，包括Wnt信号传导。后者在慢性粒细胞白血病的进展中具有独特的作用。活化的信号传导导致建立了另外的源自粒细胞巨噬细胞祖细胞的白血病干细胞群体。sFRP1是Wnt信号的抑制剂，其表达对于造血干细胞的分化和维持很重要。在这项研究中，我们旨在研究通过共诱导K562细胞中sFRP1的表达而靶向Wnt信号的miRNA。我们将详细分析hsa-mir-221-3p，hsa-mir-222-3p，hsa-mir-106b-5p，hsa-let-7f-5p，hsa-mir-182-5p，hsa-mir- 191-5p和hsa-mir-183-5p及其靶基因及其与Wnt信号传导的关系。