Chemotherapy-induced peripheral neuropathy (CIPN) manifests as mechanical allodynia and hyperalgesia, and is one of the main adverse effects of chemotherapeutic agents. Currently available therapeutic drugs are not sufficiently effective for the management of this adverse effect in the clinic. Therefore, the development of novel therapeutic agents for treating CIPN is necessary. Our previous study suggested the potential of aucubin and pedicularis-lactone (1) as active compounds responsible for the anti-allodynic property of Plantaginis Semen. However, the activity of purified 1 has not been evaluated due to its low content in Plantaginis Semen. In the present study, 1 was isolated from Viticis Fructus, as well as viteoid I (2) and viteoid II (3) during the process of isolation. The purities of isolated 1, 2, and 3 were determined as 67.15%, 92.12%, and 86.72%, respectively, by quantitative ¹H-NMR, using DSS-d₆ as an internal standard. Repeated daily oral administration of these three iridoids at a dose of 15 mg/kg significantly inhibited the PTX-induced mechanical allodynia in mice, suggesting the anti-allodynic activities of 1, 2, and 3. This study provides confirmatory evidence for the anti-allodynic activity of purified 1 and also reveals two additional active iridoids from Viticis Fructus. These three iridoids could be potential candidates for the treatment of CIPN.

化疗引起的周围神经病变（CIPN）表现为机械性异常性疼痛和痛觉过敏，是化学治疗药物的主要不良反应之一。当前可用的治疗药物对于在临床中解决这种不良作用还不够有效。因此，开发用于治疗CIPN的新型治疗剂是必要的。我们以前的研究表明，洋红花和紫杉内酯（1）作为负责车前草的抗异常性疼痛作用的活性化合物的潜力。但是，由于在Plantaginis Semen中含量低，因此未评估纯化的1的活性。在本研究中，1分离自Viticis Fructus和viteoid I（2）和类黄酮II（3）在分离过程中。的分离的纯度1，2，和3被分别确定为67.15％，92.12％，86.72和％，通过定量¹ H-NMR，使用DSS- d ₆作为内标。在剂量为15重复这三个环烯醚萜的每日口服毫克/公斤显著抑制了小鼠中的PTX诱导的机械异常性疼痛，提示的抗异常性疼痛活性1，2，和3。这项研究为纯化的1的抗异常疼痛活性提供了验证性证据。并且还揭示了来自Viticis Fructus的另外两种活性虹彩。这三个虹彩可能是治疗CIPN的潜在候选者。