Tyrosol β-galactoside (TG) is a phenylethanoid glycoside with proven neuroprotective properties. This work deals with its biocatalytic production from tyrosol and lactose using Aspergillus oryzae β-galactosidase in immobilized form. Six commercial carriers were examined to find the optimal biocatalyst. Besides standard biocatalyst performance characteristics, adsorption of the hydrophobic substrate on immobilization carrier matrices was also investigated. The adsorption of tyrosol was significant, but it did not have adverse effects on TG production. On the contrary, TG yield was improved for some biocatalysts. A biocatalyst prepared by covalent binding of β-galactosidase on an epoxy-activated carrier was used for detailed investigation of the effect of reaction conditions on glycoside production. Temperature had a surprisingly weak effect on the overall process rate. A lactose concentration of 0.83 M was found to be optimal to enhance TG formation. The impact of tyrosol concentration was rather complex. This substrate caused inhibition of all reactions. Its concentration had a strong effect on the hydrolysis of lactose and all products. Higher tyrosol concentrations, 30–40 g/L, were favorable as pseudo-equilibrium concentrations of TG and galactooligosaccharide were reached. Repeated batch results revealed excellent operational stability of the biocatalyst.

酪醇 β-半乳糖苷 (TG) 是一种苯乙醇苷，具有经证实的神经保护特性。这项工作涉及使用米曲霉从酪醇和乳糖中生物催化生产固定化形式的 β-半乳糖苷酶。检查了六种商业载体以找到最佳的生物催化剂。除了标准的生物催化剂性能特征外，还研究了疏水性底物在固定载体基质上的吸附。酪醇的吸附显着，但对TG的产生没有不利影响。相反，某些生物催化剂的 TG 产率有所提高。通过将 β-半乳糖苷酶共价结合在环氧活化载体上制备的生物催化剂用于详细研究反应条件对糖苷生产的影响。温度对整体加工速率的影响出人意料地微弱。发现 0.83 M 的乳糖浓度是增强 TG 形成的最佳选择。酪醇浓度的影响相当复杂。该底物导致所有反应的抑制。它的浓度对乳糖和所有产品的水解有很强的影响。较高的酪醇浓度（30-40 g/L）是有利的，因为达到了 TG 和低聚半乳糖的假平衡浓度。重复批次结果显示生物催化剂具有优异的操作稳定性。