We previously reported a monoclonal antibody (mAb), 1G8, against the neuraminidase (NA) of H9N2 avian influenza virus (AIV) with significant NA inhibitory activity. To generate a recombinant chickenized mAb (RCmAb) against the NA of H9N2 AIV for passive immunization in poultry, the gene of the fragment of antigen binding (Fab) of mAb 1G8 was cloned and fused with the fragment crystallizable (Fc) gene of chicken IgY. The RCmAb 1G8 was expressed in COS-1 cells and could be detected in cell culture supernatant. The results of NA inhibitory activity tests of the RCmAb 1G8 in an enzyme-linked lectin assay (ELLA) and a microneutralization (MN) assay showed that the RCmAb 1G8 maintained significant NA inhibitory activity and neutralizing ability. This is the first chickenized antibody against AIV, which would be a good candidate for passive immunization in poultry.

中文翻译：

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抗 H9N2 禽流感病毒神经氨酸酶的重组鸡化单克隆抗体的产生。


我们之前报道了一种针对 H9N2 禽流感病毒（AIV）神经氨酸酶（NA）的单克隆抗体（mAb）1G8，具有显着的 NA 抑制活性。为了产生针对 H9N2 AIV NA 的重组鸡化单克隆抗体 (RCmAb)，用于家禽被动免疫，克隆了单克隆抗体 1G8 的抗原结合片段 (Fab) 基因，并将其与鸡 IgY 的可结晶片段 (Fc) 基因融合。 RCmAb 1G8 在 COS-1 细胞中表达，并且可以在细胞培养上清液中检测到。酶联凝集素测定(ELLA)和微中和(MN)测定中RCmAb 1G8的NA抑制活性测试结果表明，RCmAb 1G8保持了显着的NA抑制活性和中和能力。这是第一个针对 AIV 的鸡源化抗体，它将成为家禽被动免疫的良好候选者。