The present study aimed to determine the phenolic compounds of Aristolochia longa root extracts and to evaluate their antibacterial activities on multiresistant strains. Phytochemical analysis revealed the presence of flavonoids, tannins, terpenoids, and alkaloids. The HPLC-DAD analysis of A. longa extracts showed the presence of several major bioactive compounds such as ferulic acid, 4-hydroxycinnamic acid, citric acid, and quinic acid. The agar diffusion method was used for the sensitivity test, while minimal inhibitory concentration (MIC) and minimal bactericidal concentration values were determined by microdilution assay. Different tests were carried out on 3 clinical multiresistant strains and 3 reference strains. The diameter of inhibition of Staphylococcus aureus ATCC 25923 induced by the ethyl acetate fraction at 200 mg/mL was 25 ± 1 mm. Moreover, Escherichia coli ATCC 29522 showed a great sensitivity toward all the concentrations tested. The MICs of the active extracts vary between 12.5 and 100 mg/mL with a bacteriostatic effect on Pseudomonas aeruginosa ATCC 27853, Enterococcus faecalis, and S. aureus ATCC 25923.

本研究旨在确定长马兜铃根提取物中的酚类化合物，并评估其对多重耐药菌株的抗菌活性。植物化学分析表明，类黄酮，单宁，萜类和生物碱的存在。long.a提取物的HPLC-DAD分析表明，存在几种主要的生物活性化合物，例如阿魏酸，4-羟基肉桂酸，柠檬酸和奎尼酸。琼脂扩散法用于敏感性测试，而微量稀释法测定最小抑菌浓度（MIC）和最小杀菌浓度值。对3种临床多耐药菌株和3种参考菌株进行了不同的测试。抑制金黄色葡萄球菌的直径由200mg / mL的乙酸乙酯级分诱导的ATCC 25923为25±1mm。此外，大肠杆菌ATCC 29522对所有测试浓度都显示出很高的敏感性。活性提取物的MIC值在12.5至100 mg / mL之间变化，对铜绿假单胞菌ATCC 27853，粪肠球菌和金黄色葡萄球菌ATCC 25923具有抑菌作用。