Human mesenchymal stem cells derived from adipose tissue (hADMSCs) are a desirable candidate in regenerative medicine. hADMSCs secrete growth factors, cytokines, and chemokines and also express various receptors that are important in cell activation, differentiation, and migration to injured tissue. We showed that the expression level of chemokine receptor CXCR6 was significantly increased by ~2.5-fold in adipogenic-differentiated cells (Ad), but not in osteogenic-differentiated cells (Os) when compared with hADMSCs. However, regulation of CXCR6 expression on hADMSCs by using lentiviral particles did not affect the differentiation potential of hADMSCs. Increased expression of CXCR6 on Ad was mediated by both receptor recycling, which was in turn regulated by secretion of CXCL16, and de novo synthesis. The level of soluble CXCL16 was highly increased in both Ad and Os in particular, which inversely correlates with the expression on a transmembrane-bound form of CXCL16 that is cleaved by disintegrin and metalloproteinase. We concluded that the expression of CXCR6 is regulated by receptor degradation or recycling when it is internalized by interaction with CXCL16 and by de novo synthesis of CXCR6. Overall, our study may provide an insight into the molecular mechanisms of the CXCR6 reciprocally expressed on differentiated cells from hADMSCs.

中文翻译：

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CXCR6在脂肪细胞和成骨细胞与人脂肪组织衍生的间充质干细胞分化后的表达调控。

源自脂肪组织的人类间充质干细胞（hADMSCs）是再生医学中理想的候选药物。hADMSC分泌生长因子，细胞因子和趋化因子，并且还表达在细胞活化，分化和迁移至受损组织中很重要的各种受体。我们显示，与hADMSCs相比，趋化因子受体CXCR6的表达水平在成脂分化的细胞（Ad）中显着增加了约2.5倍，而在成骨分化的细胞（Os）中却没有增加。但是，使用慢病毒颗粒调节hADMSCs上CXCR6的表达不会影响hADMSCs的分化潜能。CXCR6在Ad上的表达增加是由受体循环（由CXCL16的分泌和从头合成）调节的。可溶性CXCL16的水平在Ad和Os中都特别增加，这与CXCL16跨膜结合形式的表达成反比，该形式被整合素和金属蛋白酶切割。我们得出结论，当CXCR6的表达通过与CXCL16的相互作用和CXCR6的从头合成而被内在化时，受受体降解或再循环的调节。总的来说，我们的研究可能提供对从hADMSCs分化的细胞上相互表达的CXCR6分子机制的见解。我们得出结论，当CXCR6的表达通过与CXCL16的相互作用和CXCR6的从头合成而被内在化时，受受体降解或再循环的调节。总的来说，我们的研究可能提供对从hADMSCs分化的细胞上相互表达的CXCR6分子机制的见解。我们得出结论，当CXCR6的表达通过与CXCL16的相互作用和CXCR6的从头合成而被内在化时，受受体降解或再循环的调节。总的来说，我们的研究可能提供对从hADMSCs分化的细胞上相互表达的CXCR6分子机制的见解。