SummaryThe seminal characteristics of Moenkhausia oligolepis are described. Three males were induced with a single dose of carp pituitary. Semen was collected 6 h after induction, and diluted in dibasic sodium phosphate extender solution. For motility analysis, 1 µl of diluted semen was added to 10 µl of distilled water to achieve gamete activation. The average duration of total motility was 76.67 s; while the average sperm motility rate at intervals of 15 s was 95.3, 85.3, 59.6, 31.7, 13.0, 4.6 and 1.2%. To determine sperm concentration in samples, 0.5 μl of semen was diluted with 500 μl of glutaraldehyde. An aliquot of 10 μl of this dilution was utilized for cell counting. An average count of 4.97 × 109 ± 3.46 sperm/ml was obtained. Morphological analyses were performed using eosin–nigrosine dye; 20.33% of the sperm were observed to be dead. Live sperm, comprising the other 79.67%, had an average length of approximately 30 µm, with a head diameter of 4.488 ± 0.7 µm; and a flagella plus mid-piece length of 26.071 ± 12.4 µm. Of those sperm, 69% had a normal morphology, while 31% had primary and secondary abnormalities. The observed abnormality rate did not have a detrimental effect on artificial fertilization potential for the species. The description of the seminal characteristics of a species is one of the most important sets of information required for artificial reproduction of fish in captivity. It also contributes significantly to the total biological knowledge of the studied species.

中文翻译：

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亚马逊火眼 tetra Moenkhausia oligolepis (Günther, 1864) 的开创性特征

总结的开创性特征鳐鱼被描述。用单剂量的鲤鱼垂体诱导三名雄性。诱导后6小时收集精液，用磷酸氢二钠稀释液稀释。对于运动性分析，将 1 µl 稀释精液添加到 10 µl 蒸馏水中以实现配子激活。总运动平均持续时间为 76.67 秒；而15 s间隔的平均精子活力分别为95.3、85.3、59.6、31.7、13.0、4.6和1.2%。为了确定样本中的精子浓度，将 0.5 μl 精液用 500 μl 戊二醛稀释。将该稀释液的 10 μl 等分试样用于细胞计数。平均计数为 4.97 × 109获得 ± 3.46 精子/毫升。使用曙红-苯胺黑染料进行形态学分析；观察到 20.33% 的精子死亡。其余 79.67% 的活精子平均长度约为 30 µm，头部直径为 4.488 ± 0.7 µm；鞭毛加中段长度为 26.071 ± 12.4 µm。在这些精子中，69% 的精子形态正常，而 31% 的精子有原发性和继发性异常。观察到的异常率对该物种的人工受精潜力没有不利影响。对一个物种的基因特征的描述是人工繁殖人工繁殖圈养鱼类所需的最重要的信息集之一。它还对所研究物种的总体生物学知识做出了重大贡献。